Nitrogen fixation potential and agronomic evaluation of three hypernodulating mutants of soybean

The three hypernodulating mutants (NOD1-3, NOD2-4, and NOD3-7) of soybean (Glycine max (L.) Merr.) and the parent (Williams) were evaluated for short-term NO$\sb3\sp-$ effect on N$\sb2$ fixation, measured by an acetylene (C$\sb2$H$\sb2$) reduction assay and by the relative ureide abundance technique. Nitrate was supplied to 3-week-old, hydroponically grown plants with fully developed nodules. Application of 5 mM NO$\sb3\sp-$ resulted in inhibition of nitrogen fixation of all soybean lines, Williams being most affected. Of all mutants tested, NOD1-3 was most tolerant of NO$\sb3\sp-$ in terms of C$\sb2$H$\sb2$ reduction activity, and also showed highest relative ureide abundance in dry tissue extracts. It was found, however, that the C$\sb2$H$\sb2$ reduction activity per gram of nodules was lower for NOD1-3 than for Williams, in both the presence and absence of NO$\sb3\sp-$. NOD1-3 had higher nodule ureide concentration and had similar glutamine synthetase activity in nodule tissue. Nitrate nutrition was also found to result in ureide accumulation in all plant parts of both NOD1-3 and Williams. The data obtained from young plants indicated that although the hypernodulating mutants of soybean possess appreciable NO$\sb3\sp-$ tolerance in terms of nodule initiation and formation, N$\sb2$ fixation was still inhibited by the presence of NO$\sb3\sp-$.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Wu, Shenchuan

Contributors dc:contributor

• Harper, James E.

• Agriculture
• Biology
• Plant Physiology
• Agronomy

Statement dc:rights

• Copyright 1990 Shenchuan Wu

Language dc:language

eng

Identifier

AAI9026357
(UMI)AAI9026357

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/19005