Mutational analysis of integrase and excisionase proteins in bacteriophage lambda site-specific recombination system

Second-site reversion analyses were performed on 21 recombination defective Int mutants. A second-site revertant P243L/E218K and a pseudorevertant E175K were isolated and further characterized using genetic and biochemical methods. The results showed that the second-site substitution E218K selectively enhances Int core-binding affinity and acts as a global suppressor. In addition, our genetic data suggest that position 218 is part of or near the putative Int core-binding domain.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Wu, Zhao

Contributors dc:contributor

• Gardner, Jeffrey F.
• Gumport, Richard I.
• Voss, Edward W., Jr.

• Biology, Molecular
• Biology, Microbiology

Statement dc:rights

• Copyright 1995 Wu, Zhao

Language dc:language

eng

Identifier

AAI9624540
(UMI)AAI9624540

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/18938