Quantitative phase imaging for blood cytometry

Blood smear analysis has remained a crucial diagnostic tool for pathologists despite the advent of automatic analyzers such as flow cytometers and impedance counters. Though these current methods have proven to be indispensable tools for physicians and researchers alike, they provide limited information on the detailed morphology of individual cells and merely alert the operator to manually examine a blood smear by raising flags when abnormalities are detected. Here I demonstrate an automatic interferometry based smear analysis technique known as Diffraction Phase Cytometry (DPC), which is capable of providing the same information on red blood cells as is provided by current clinical analyzers, while rendering additional, currently unavailable parameters on the 2D and 3D morphology of individual red blood cells (RBCs). To validate the utility of the technique in a clinical setting, I present a comparison between tests generated from 32 patients by a state-of-the-art clinical impedance counter and DPC. The majority of work presented in this thesis is drawn from two publications: M. Mir et al., Journal of Biomedical Optics, vol. 15 (2), 2010, and M. Mir et al. Optics Express, vol. 17, 2009.

Name thesis:degree_name

M.S.

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Electrical & Computer Engr

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2011

Author dc:creator

• Mir, Mustafa A.

Contributors dc:contributor

• Popescu, Gabriel

• Quantitative Phase Imaging
• Blood Cytometry
• Interferometry
• Single Cell Morphology

Statement dc:rights

• Copyright 2010 Mustafa A. Mir

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/18321

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/18321