High-throughput detection of protein—protein and protein—DNA interactions via photonic crystal biosensors: applications to apoptosis inducing factor

High-throughput screening (HTS) has played an integral role in the discovery of lead molecules that eventually are transformed into the therapies of tomorrow. Contributing towards the success of any screening campaign is the target choice, chemical library size and diversity, as well as the technology utilized in screening. Photonic crystal (PC) biosensors are a relatively new technology whose potential is only beginning to be realized. PC biosensors are a “labelfree” technology compatible with standard high-throughput techniques. Macromolecular interactions including protein—small molecule, protein—nucleic acid, protein—protein, and whole cell interactions can be measured using PC biosensors. Presented herein is the first application of PC biosensors to a protein—nucleic acid interaction and a simplified protein— protein interaction platform. The protein—nucleic acid interaction targeted is the apoptosis inducing factor (AIF)—DNA interaction, which is critical for the mediation of caspaseindependent cell death in neurodegenerative disease and stroke. Protein—protein interactions were targeted by modifying the PC biosensor surface for detection of the His6 tag, which is widely employed in protein purification.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2010

Author dc:creator

• Heeres, James T.

Contributors dc:contributor

• Hergenrother, Paul J.
• Cunningham, Brian T.
• Shapiro, David J.
• Morrissey, James H.

• Photonic Crystals
• Apoptosis Inducing Factor
• apoptosis inducing factor (AIF)
• Protein-DNA Interactions
• Protein-Protein Interactions

Statement dc:rights

• Copyright 2010 James T. Heeres

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/17028

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/17028