Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 48 for “"DNA interaction"”.
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Protein-DNA interaction, random walks and polymer statistics
… framework describing the kinetics of protein- DNA interaction is developed. Recognition and binding of specific sites on DNA by proteins is central for many cellular functions such as transcription, replication, and recombination. In the process of recognition, a protein rapidly searches for …
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A Mutational Analysis of Bacteriophage Lambda Site-Specific Recombination (In Vitro, Dna-Dna Interaction, Protein, Oligonucleotide-Directed)
… homology demonstrated that homology dependent DNA-DNA interactions only occur within the 7-base "overlap" region where strand exchange occurs. In vitro recombination analyses with mutant att sites further demonstrated that the homology dependence involves DNA interactions between one strand in …
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SWI/SNF-NUCLEOSOME INTERACTIONS AND DISASSEMBLY OF NUCLEOSOMES: NOVEL METHODOLOGIES FOR MAPPING PROTEIN-PROTEIN AND PROTEIN-DNA INTERACTION
… University Carbondale. TITLE: SWI/SNF-NUCLEOSOME INTERACTIONS AND DISASSEMBLY OF NUCLEOSOMES: NOVEL METHODOLOGIES FOR MAPPING PROTEIN-PROTEIN AND PROTEIN-DNA INTERACTION MAJOR PROFESSOR: Dr. Blaine Bartholomew The SWI/SNF complex disrupts and mobilizes chromatin in an ATP-dependent manner. A …
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Multi Scale Fluorescence Studies: The Spinning Disk Confocal Fluorescence Lifetime Imaging Microscopy & Single Molecule Nano-Metronome & Single Molecule Study of Tetramethyrhodamine -Dna Interaction
… (TMR) attached to the end of DNA oligomer. The study reveals that in the absence of any biological activities of DNA, the fluorescence emission of TMR undergoes multiple slow transitions among at least four different fluorescence states. While the actual number of fluorescence …
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Multi Scale Fluorescence Studies: The Spinning Disk Confocal Fluorescence Lifetime Imaging Microscopy &Amp; Single Molecule Nano-Metronome &Amp; Single Molecule Study of Tetramethyrhodamine-Dna Interaction
… the FLIM setup is used to study the close interactions between structural proteins in the body wall muscle cells of the nematode Caenorhabditis elegans and visualize the activation states of a signaling protein in cultured neurons of Drosophila melanogaster based on the change in the …
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NONCOVALENT INTERACTION OF PLATINUM PLANAR AMINE COMPOUNDS WITH TRYPTOPHAN: A STRATEGY TO INTERFERE WITH P53-MDM2 INTERACTIONS AND TARGETING RETROVIRAL ZN FINGER-DNA INTERACTION (HIV NCP7)
<p>Non-covalent interactions involving π-π stacking play an essential role in self-assembly and molecular recognition processes such as protein folding and DNA/RNA-protein selective recognition. The knowledge gained from these studies could provide insight into possible site recognition complexes, …
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Computational Inference of Genome-Wide Protein-DNA Interactions Using High-Throughput Genomic Data
… One important aspect of this regulation is the interaction between regulatory proteins, such as transcription factors (TF) and nucleosomes, and the genome. Different high-throughput techniques have been invented to map these interactions genome-wide, including ChIP-based methods (ChIP-chip, …
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Statistical thermodynamics of virus assembly
… of MgSO4 concentration on the ejection of DNA from bacteriophage lambda. There is a concentration, N0, at which the minimum amount of DNA is ejected. At lower or higher concentrations, more DNA is ejected. We propose that this non-monotonic behavior is due to the overcharging of DNA at high …
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Structural basis for the function of the transcriptional regulator KstR from Mycobacterium tuberculosis
… targeting the cholesterol metabolism in Mtb. The interactions between KstR and cognate DNA or ligands have been investigated by multiple biochemical techniques (i.e. electrophoretic mobility shift assay, surface plasmon resonance and intrinsic fluorescence quenching assay). Protein-DNA binding …
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Tuning the Photophysical and Biological Properties of a Series of Ruthenium-Based Chromophores and Chromophore Coupled Cisplatin Analogs with Substituted Terpyridine Ligands
… modifications on spectroscopic properties, DNA interaction, and bioactivity of tridentate, terpyridine containing ruthenium-based chromophores and chromophore coupled cisplatin analogs. The coupling of a light absorbing unit to a bioactive site offers the potential for developing …
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Structural Studies of DNA Replication Machinery in Archaea
DNA replication is a fundamental process present in all living organisms. It is the means by which organisms transfer their genetic information to their offspring to ensure the propagation of their lineage. One of the essential features of DNA replication is to separate the DNA double helix to …
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Dynamic Simulation of active/inactive Chromatin Domains
… agent (HMG/SAR, HP1, cohesin, condensin, DNA-DNA interaction...) can be modelled as an effective attractive potential of specific chain segments. This way the formation of individual 1Mbp sized rosettes from a linear chain could be observed. Furthermore several Mbp of fiber were simulated, …
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High-Throughput Detection of Protein---Protein and Protein---Dna Interactions via Photonic Crystal Biosensors: Applications to Apoptosis Inducing Factor
… high-throughput techniques. Macromolecular interactions including protein---small molecule, protein---nucleic acid, protein---protein, and whole cell interactions can be measured using PC biosensors. Presented herein is the first application of PC biosensors to a protein---nucleic acid …
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High-throughput detection of protein—protein and protein—DNA interactions via photonic crystal biosensors: applications to apoptosis inducing factor
… high-throughput techniques. Macromolecular interactions including protein—small molecule, protein—nucleic acid, protein—protein, and whole cell interactions can be measured using PC biosensors. Presented herein is the first application of PC biosensors to a protein—nucleic acid interaction …
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Cytochrome c-DNA and cytochrome c-enzyme interactions for the construction of analytical signal chains
… surfaces. With the help of the biopolymer DNA, the redox protein assembles into electro active multilayer (ML) systems, providing a biocompatible matrix for the entrapment of proteins. In this study the characteristics of the cyt c and DNA interaction were defined on the molecular level for …
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High-Throughput Screening Development Using Photonic-Crystal Optical Biosensor
Protein-DNA interactions are essential for fundamental cellular processes such as transcription, DNA damage repair, and apoptosis. As such, small molecule disruptors of these interactions could be powerful tools for investigation of these biological processes, and such compounds would have great …
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Comparative analysis of the promoters of the CyI-CyIIa-CyIIb actin gene cluster in the sea urchin Strongylocentrotus purpuratus
… was subcloned and sequenced. Sites of protein-DNA interaction were identified using DNaseI footprinting. Comparisons of this data with that previously obtained for CyI and CyIIb showed that while CyI and CyIIb share an identical CArG (or serum response element) and extensive sequence homology …
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Attempts to localize the replicative form of polyoma virus DNA in mouse embryo cells
In order to localize replicating polyoma viral DNA (Py II*), a nuclear extraction technique is chosen and then slightly modified in order to rapidly yield purified nuclei in which the polyoma viral (Py) DNA is intact. A method is also developed which breaks the nuclear membrane, but not the Py DNA …
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Systematic determination of a transcription factor/binding site functional interaction landscape
… of regulatory proteins that specifically bind DNA to modulate the expression of targeted genes. While they have been extensively studied, major questions remain about the protein-DNA interaction underlying this hub of regulation. What binding site sequences functionally interact with a given …
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A Statistical Model to Determine Multiple Binding Sites of a Transcription Factor on DNA Using ChIP-seq Data
<p>Protein-DNA interaction is vital to many biological processes in cells such as cell division, embryo development and regulating gene expression. Chromatin Immunoprecipitation followed by massively parallel sequencing (ChIP-seq) is a new technology that can reveal protein binding sites in genome …
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