Single molecule study of weak and transient biological interactions

Over the past decade, major breakthroughs were led in molecular biology with techniques allowing the study of dynamics at the resolution of an individual molecule. We have been expanding this capability by adapting porous vesicle encapsulation to single molecule fluorescence techniques. The porous vesicle encapsulation method allows the study of individual macromolecules in a biofriendly, cell-like environment, where the chemical condition can be changed and the desired reactions are triggered at will. Specifically, this adaptation now allows the study of weakly and transiently interacting biomolecules at the single-molecule resolution. Here we present unique interactions between proteins and nucleic acids which were previously unattainable with conventional single molecule techniques.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Physics

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2010

Author dc:creator

• Cisse, Ibrahim

Contributors dc:contributor

• Ha, Taekjip
• Chemla, Yann R.
• Goldenfeld, Nigel D.
• Stack, John D.

• Protein-Protein Interactions
• Single Molecule
• Weak Interactions
• Transient Interactions
• Protein-DNA Interactions
• DNA-DNA Interactions
• RNA-RNA Interactions

Statement dc:rights

• Copyright 2009 Ibrahim Cisse

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/14744

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/14744