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Specifically, this adaptation now allows the study of weakly and transiently interacting biomolecules at the single-molecule resolution. Here we present unique interactions between proteins and nucleic acids which were previously unattainable with conventional single molecule techniques.","abstract_html":"Over the past decade, major breakthroughs were led in molecular biology with techniques allowing the study of dynamics at the resolution of an individual molecule. We have been expanding this capability by adapting porous vesicle encapsulation to single molecule fluorescence techniques. The porous vesicle encapsulation method allows the study of individual macromolecules in a biofriendly, cell-like environment, where the chemical condition can be changed and the desired reactions are triggered at will. Specifically, this adaptation now allows the study of weakly and transiently interacting biomolecules at the single-molecule resolution. Here we present unique interactions between proteins and nucleic acids which were previously unattainable with conventional single molecule techniques.","abstract_has_math":false,"creators":["Cisse, Ibrahim"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Physics","degree_department":null,"school":null,"contributors":["Ha, Taekjip","Chemla, Yann R.","Goldenfeld, Nigel D.","Stack, John D."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2010,"date_issued":"2010-01-06T17:49:07Z","date_published":"2010-01-06T17:49:07Z","updated_at":"2026-07-22T22:25:08Z","subjects":["Protein-Protein Interactions","Single Molecule","Weak Interactions","Transient Interactions","Protein-DNA Interactions","DNA-DNA Interactions","RNA-RNA Interactions"],"languages":["en"],"rights":["Copyright 2009 Ibrahim Cisse"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/14744","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Ha, Taekjip","Chemla, Yann R.","Goldenfeld, Nigel D.","Stack, John D."]},{"key":"dc:creator","label":"Author","values":["Cisse, Ibrahim"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2010-01-06T17:49:07Z","2012-01-07T11:00:11Z","2009-12"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Physics"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Protein-Protein Interactions","Single Molecule","Weak Interactions","Transient Interactions","Protein-DNA Interactions","DNA-DNA Interactions","RNA-RNA Interactions"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2009 Ibrahim Cisse"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/14744"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Over the past decade, major breakthroughs were led in molecular biology with techniques allowing the study of dynamics at the resolution of an individual molecule. 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