Studies of protein folding on membranes and in crowded environments and bridging the research-teaching gap in K-12 science

This work deals with three important problems in membrane protein folding studies, namely the preparation and storage of homogeneous small unilamellar vesicles (SUV), the development of an algorithm for selecting soluble trans-membrane helices from known membrane proteins, and the characterization of membrane binding of single surface and trans-membrane helices. I then describe the effects of excluded volume on the stability and kinetics of a stable Lambda Repressor mutant Y22WQ33YA3749G. Finally, I discuss two education projects that aim to bridge the gap between scientific research and K-12 teaching.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biophysics & Computnl Biology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2010

Author dc:creator

• Denos, Sharlene

Contributors dc:contributor

• Gruebele, Martin
• Martin Gruebele
• Clegg, Robert M.
• Crofts, Antony R.
• Gennis, Robert B.

• Proteins
• Protein Folding
• Crowding
• Science Education
• Science Outreach
• Membrane Proteins
• Peptide Design

Statement dc:rights

• Copyright 2009 Sharlene Denos

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/14567

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/14567