{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/14567"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/14567","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Studies of protein folding on membranes and in crowded environments and bridging the research-teaching gap in K-12 science","abstract":"This work deals with three important problems in membrane protein folding studies, namely the preparation and storage of homogeneous small unilamellar vesicles (SUV), the development of an algorithm for selecting soluble trans-membrane helices from known membrane proteins, and the characterization of membrane binding of single surface and trans-membrane helices. I then describe the effects of excluded volume on the stability and kinetics of a stable Lambda Repressor mutant Y22WQ33YA3749G. Finally, I discuss two education projects that aim to bridge the gap between scientific research and K-12 teaching.","abstract_html":"This work deals with three important problems in membrane protein folding studies, namely the preparation and storage of homogeneous small unilamellar vesicles (SUV), the development of an algorithm for selecting soluble trans-membrane helices from known membrane proteins, and the characterization of membrane binding of single surface and trans-membrane helices. I then describe the effects of excluded volume on the stability and kinetics of a stable Lambda Repressor mutant Y22WQ33YA3749G. Finally, I discuss two education projects that aim to bridge the gap between scientific research and K-12 teaching.","abstract_has_math":false,"creators":["Denos, Sharlene"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Biophysics & Computnl Biology","degree_department":null,"school":null,"contributors":["Gruebele, Martin","Martin Gruebele","Clegg, Robert M.","Crofts, Antony R.","Gennis, Robert B."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2010,"date_issued":"2010-01-06T16:12:35Z","date_published":"2010-01-06T16:12:35Z","updated_at":"2026-07-22T22:25:07Z","subjects":["Proteins","Protein Folding","Crowding","Science Education","Science Outreach","Membrane Proteins","Peptide Design"],"languages":["en"],"rights":["Copyright 2009 Sharlene Denos"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/14567","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Gruebele, Martin","Martin Gruebele","Clegg, Robert M.","Crofts, Antony R.","Gennis, Robert B."]},{"key":"dc:creator","label":"Author","values":["Denos, Sharlene"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2010-01-06T16:12:35Z","2009-12"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biophysics & Computnl Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Proteins","Protein Folding","Crowding","Science Education","Science Outreach","Membrane Proteins","Peptide Design"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2009 Sharlene Denos"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/14567"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["This work deals with three important problems in membrane protein folding studies, namely the preparation and storage of homogeneous small unilamellar vesicles (SUV), the development of an algorithm for selecting soluble trans-membrane helices from known membrane proteins, and the characterization of membrane binding of single surface and trans-membrane helices. I then describe the effects of excluded volume on the stability and kinetics of a stable Lambda Repressor mutant Y22WQ33YA3749G. Finally, I discuss two education projects that aim to bridge the gap between scientific research and K-12 teaching.","Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2009-12-03T00:58:24Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Denos_Sharlene.pdf: 57584178 bytes, checksum: cf7c6d68308bd34042c49ff5285ddb05 (MD5)","Made available in DSpace on 2010-01-06T16:12:35Z (GMT). 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I then describe the effects of excluded volume on the stability and kinetics of a stable Lambda Repressor mutant Y22WQ33YA3749G. Finally, I discuss two education projects that aim to bridge the gap between scientific research and K-12 teaching.","Item withdrawn by Mark Zulauf (zulauf@illinois.edu) on 2009-12-03T00:58:24Z Item was in collections: University of Illinois Theses & Dissertations (ID: 1) No. of bitstreams: 1 Denos_Sharlene.pdf: 57584178 bytes, checksum: cf7c6d68308bd34042c49ff5285ddb05 (MD5)","Made available in DSpace on 2010-01-06T16:12:35Z (GMT). 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