Hydrothermal synthesis and characterization of carbon dots for enhanced biosensing: A focus on optical properties and DNA interactions

Carbon dots (CDs) have emerged as a promising class of nanomaterials for biosensing applications due to their unique optical properties, including high fluorescence quantum yields, photostability, and biocompatibility. This thesis investigates the synthesis, characterization, and application of CDs in biosensing, focusing on the fluorescence quenching phenomena associated with single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). The synthesis of CDs was optimized using various carbon precursors and functionalization agents to enhance their fluorescence properties. The interaction dynamics between CDs and nucleic acids were analyzed through concentration, time and structure dependent studies, revealing that these parameters significantly affect fluorescence intensities with quenching effects. The study further explores possible utilization of CDs in real-time biosensing, highlighting their potential for use in point- of-care diagnostics. Findings indicate that the CDs can effectively bind to nucleic acids while maintaining fluorescence, suggesting their versatility in detecting various molecular targets. This research contributes to the understanding of the behavior of CDs in biological systems and underscores their significance as effective fluorescent probes for viral detection.

Name thesis:degree_name

M.S.

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Bioengineering

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2024

Author dc:creator

• Morshed, Mehzabin

Contributors dc:contributor

• Wang, Xing

• Carbon Dots (cds)
• Biosensing Applications
• Fluorescence Quenching
• Optical Properties
• Nucleic Acid Interactions
• Point-of-care Diagnostics
• Real-time Biosensing
• Carbon Precursor Optimization
• Functionalization Agents
• Viral Detection
• Dna Structure-dependent Quenching

Statement dc:rights

• Copyright 2024 Mehzabin Morshed

Language dc:language

eng, en

Handle dc:identifier

https://hdl.handle.net/2142/127358

OAI record oai:identifier

https://www.ideals.illinois.edu/oai-pmh?verb=GetRecord&metadataPrefix=etdms&identifier=oai%3Awww.ideals.illinois.edu%3A2142%2F127358