Circadian rhythmicity of rat neuroendothelial cells at the blood-brain interface

The Blood-Brain Interface (BBI) facilitates neurological protection from pathogens, the removal of waste products, brain homeostasis, and the permeation of nutrients and drugs. Its dysfunction contributes to the onset of a variety of neurological diseases, including strokes and dementia. Research has shown that both BBI permeability and human susceptibility to strokes fluctuate according to time of day. However, more in vitro studies are required to isolate the effects of circadian rhythms from the effects of sleep and other confounding factors on BBI permeability. The role of neuroendothelial cells, a key component of the BBI, has also not been fully elucidated. We have determined that barrier tightness and permeability oscillate according to time of day in rat neuroendothelial cells. These findings extended to permeability to the blood clotting factor fibrinogen. We also determined that the addition of Carbachol, a pan-acetylcholine receptor agonist, could shift these rhythms.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Molecular & Integrative Physi

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2024

Author dc:creator

• Weiss, Amanda C

Contributors dc:contributor

• Gillette, Martha U
• Kong, Hyunjoon
• Chung, Hee Jung
• Sweedler, Jonathan V

• Blood-brain Barrier
• Circadian Rhythms
• Blood-brain Interface
• Blood-brain Barrier Permeability

Statement dc:rights

• Copyright 2024 Amanda Weiss

Language dc:language

en, eng

Handle dc:identifier

https://hdl.handle.net/2142/125600

OAI record oai:identifier

https://www.ideals.illinois.edu/oai-pmh?verb=GetRecord&metadataPrefix=etdms&identifier=oai%3Awww.ideals.illinois.edu%3A2142%2F125600