Microfluidic biochip for optical detection of sepsis biomarkers from whole blood samples

Sepsis is a life-threatening condition with a high healthcare burden in terms of patient death and hospital readmissions. Despite its cost, sepsis remains difficult to treat due to days-long layover for lab testing as well as its nonspecific symptoms. In recent years, multiple technologies have been developed capable of detecting biomarkers of sepsis. Some technologies detect circulating proteins, others detect proteins expressed on the surface of cells (cell-surface proteins). However, detecting both types of biomarkers from a whole blood sample remains challenging. Here we propose a technology capable of doing this by combining specific capture, immunoassays and optical detection. In this Master's Thesis we have developed the protocol for the detection of C-Reactive Protein (CRP) from whole blood samples using a sandwich immunoassay and fluorescent microspheres. Additionally, we have developed the microscope setup for optical detection and demonstrated on-chip CRP detection from whole blood samples.

Name thesis:degree_name

M.S.

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Bioengineering

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2024

Author dc:creator

• Heredia, John

Contributors dc:contributor

• Valera, Enrique

• Microfluidics
• Blood
• Crp
• Creactiveprotein
• Sepsis
• Labonachip
• Optical
• Fluorescence

Statement dc:rights

• Copyright 2024 John Heredia

Language dc:language

en, eng

Handle dc:identifier

https://hdl.handle.net/2142/124434

OAI record oai:identifier

https://www.ideals.illinois.edu/oai-pmh?verb=GetRecord&metadataPrefix=etdms&identifier=oai%3Awww.ideals.illinois.edu%3A2142%2F124434