{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/124434"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/124434","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Microfluidic biochip for optical detection of sepsis biomarkers from whole blood samples","abstract":"Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-09-16 without embargo terms","abstract_html":"Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-09-16 without embargo terms","abstract_has_math":false,"creators":["Heredia, John"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Bioengineering","degree_department":null,"school":null,"contributors":["Valera, Enrique"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2024,"date_issued":"2024-05","date_published":"2024-05","updated_at":"2026-07-22T22:25:00Z","subjects":["Microfluidics","Blood","Crp","Creactiveprotein","Sepsis","Labonachip","Optical","Fluorescence"],"languages":["en","eng"],"rights":["Copyright 2024 John Heredia"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/2142/124434","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Valera, Enrique"]},{"key":"dc:creator","label":"Author","values":["Heredia, John"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2024-05","2024-05-02"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Bioengineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Microfluidics","Blood","Crp","Creactiveprotein","Sepsis","Labonachip","Optical","Fluorescence"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en","eng"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2024 John Heredia"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://hdl.handle.net/2142/124434"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-09-16 without embargo terms","The student, John Heredia, accepted the attached license on 2024-04-29 at 15:01.","The student, John Heredia, submitted this Thesis for approval on 2024-04-29 at 15:08.","This Thesis was approved for publication on 2024-05-02 at 16:27.","DSpace SAF Submission Ingestion Package generated from Vireo submission #20695 on 2024-09-16 at 00:37:23","Sepsis is a life-threatening condition with a high healthcare burden in terms of patient death and hospital readmissions. Despite its cost, sepsis remains difficult to treat due to days-long layover for lab testing as well as its nonspecific symptoms. In recent years, multiple technologies have been developed capable of detecting biomarkers of sepsis. Some technologies detect circulating proteins, others detect proteins expressed on the surface of cells (cell-surface proteins). However, detecting both types of biomarkers from a whole blood sample remains challenging. Here we propose a technology capable of doing this by combining specific capture, immunoassays and optical detection. In this Master's Thesis we have developed the protocol for the detection of C-Reactive Protein (CRP) from whole blood samples using a sandwich immunoassay and fluorescent microspheres. Additionally, we have developed the microscope setup for optical detection and demonstrated on-chip CRP detection from whole blood samples."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Microfluidic biochip for optical detection of sepsis biomarkers from whole blood samples"]}]}],"canonical_facts":{"dc:contributor":["Valera, Enrique"],"dc:creator":["Heredia, John"],"dc:date":["2024-05","2024-05-02"],"dc:description":["Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2024-09-16 without embargo terms","The student, John Heredia, accepted the attached license on 2024-04-29 at 15:01.","The student, John Heredia, submitted this Thesis for approval on 2024-04-29 at 15:08.","This Thesis was approved for publication on 2024-05-02 at 16:27.","DSpace SAF Submission Ingestion Package generated from Vireo submission #20695 on 2024-09-16 at 00:37:23","Sepsis is a life-threatening condition with a high healthcare burden in terms of patient death and hospital readmissions. Despite its cost, sepsis remains difficult to treat due to days-long layover for lab testing as well as its nonspecific symptoms. In recent years, multiple technologies have been developed capable of detecting biomarkers of sepsis. Some technologies detect circulating proteins, others detect proteins expressed on the surface of cells (cell-surface proteins). However, detecting both types of biomarkers from a whole blood sample remains challenging. Here we propose a technology capable of doing this by combining specific capture, immunoassays and optical detection. In this Master's Thesis we have developed the protocol for the detection of C-Reactive Protein (CRP) from whole blood samples using a sandwich immunoassay and fluorescent microspheres. Additionally, we have developed the microscope setup for optical detection and demonstrated on-chip CRP detection from whole blood samples."],"dc:format":["application/pdf"],"dc:identifier":["https://hdl.handle.net/2142/124434"],"dc:language":["en","eng"],"dc:rights":["Copyright 2024 John Heredia"],"dc:subject":["Microfluidics","Blood","Crp","Creactiveprotein","Sepsis","Labonachip","Optical","Fluorescence"],"dc:title":["Microfluidic biochip for optical detection of sepsis biomarkers from whole blood samples"],"dc:type":["text"],"thesis:degree_discipline":["Bioengineering"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:25:00Z"}