Protein corona formation and analysis on colloidal inorganic nanoparticles

Due to their unique physiochemical properties exhibited at the nanoscale, nanoparticles are being increasingly utilized to better human experience in many different applications, including energy, transportation, information technology, medicine, food safety, and consumer products. The widespread interest and utilization of their tunable properties will likely result in the release of nanoparticles into the environment. Additional molecular understanding of the protein corona is needed to prevent negative environmental and health impacts and to develop sustainable nanotechnology. In this work, Chapter 1 introduces the field of nanotechnology and colloidal inorganic nanoparticles with a focus on gold and iron oxide nanoparticles as well as the concepts of the nanobio interface and the importance of the protein corona. Chapter 2 details the importance of isolation method on the protein corona composition when analyzing the protein corona upon incubation with gold-coated iron oxide nanoparticles. Chapter 3 describes the consequences of protein disulfide isomerase, a redox-active protein, upon exposure to gold nanoparticles using limited proteolytic mass spectrometry analysis. In Chapter 4, the focus is shifted to understanding the effects of nanoparticle surface functionalization on the protein corona formation in plants.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2023

Author dc:creator

• Hoang, Khoi Nguyen Le

Contributors dc:contributor

• Murphy, Catherine J
• Chan, Jefferson
• Huang, Pinshane
• Wheeler, Korin E

• Protein Corona
• Inorganic Nanoparticles

Statement dc:rights

• Copyright 2023 Khoi Nguyen Hoang

Language dc:language

en, eng

Handle dc:identifier

https://hdl.handle.net/2142/121212

OAI record oai:identifier

https://www.ideals.illinois.edu/oai-pmh?verb=GetRecord&metadataPrefix=etdms&identifier=oai%3Awww.ideals.illinois.edu%3A2142%2F121212