Investigating the size effect of nanoparticles in Click Chemistry mediated cancer targeting

Size is one of the most important properties of nanoparticles (NPs) that affects their biodistribution, accumulation and retention in the tumour tissue. The effect of NP size on tumour targeting has been studied in various contexts, with or without a targeting mechanism incorporated. Herein, we report our investigation of the size effect of NPs in Click Chemistry mediated cancer targeting. Realized by anchoring NPs onto the cell surface via metabolic glycoengineering for the cell surface placement of azido group followed by Click Chemistry mediated covalent reaction between the NP and the cell, we demonstrate that the combination effect of deeper penetration of smaller-size NP coupled with reduced clearance presumably due to the Click reaction results in its higher accumulation in the tumour tissue. Specifically, we firstly used metabolic glycoengineering to introduce azido groups to the cell surface, and then investigated the accumulation profiles of dibenzocyclooctyne conjugated nanoparticles bearing different sizes (20, 50 and 200 nm). The 20-nm silica nanoconjugates (NCs) were found to have the highest accumulation in the tumour tissue both ex vivo and in vivo. This work provides further insights into the design of nanomedicine for cancer targeting when the targeting is mediated by a non-conventional, covalent-chemistry-mediated approach.

Name thesis:degree_name

M.S.

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Materials Science & Engr

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2021

Author dc:creator

• Wang, Ying

Contributors dc:contributor

• Cheng, Jianjun

• Silica Nanoparticles, Cancer targeting, metabolic engineering

Statement dc:rights

• Copyright 2021 Ying Wang

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/110842

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/110842