{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/110842"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/110842","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Investigating the size effect of nanoparticles in Click Chemistry mediated cancer targeting","abstract":"Size is one of the most important properties of nanoparticles (NPs) that affects their biodistribution, accumulation and retention in the tumour tissue. The effect of NP size on tumour targeting has been studied in various contexts, with or without a targeting mechanism incorporated. Herein, we report our investigation of the size effect of NPs in Click Chemistry mediated cancer targeting. Realized by anchoring NPs onto the cell surface via metabolic glycoengineering for the cell surface placement of azido group followed by Click Chemistry mediated covalent reaction between the NP and the cell, we demonstrate that the combination effect of deeper penetration of smaller-size NP coupled with reduced clearance presumably due to the Click reaction results in its higher accumulation in the tumour tissue. Specifically, we firstly used metabolic glycoengineering to introduce azido groups to the cell surface, and then investigated the accumulation profiles of dibenzocyclooctyne conjugated nanoparticles bearing different sizes (20, 50 and 200 nm). The 20-nm silica nanoconjugates (NCs) were found to have the highest accumulation in the tumour tissue both ex vivo and in vivo. This work provides further insights into the design of nanomedicine for cancer targeting when the targeting is mediated by a non-conventional, covalent-chemistry-mediated approach.","abstract_html":"Size is one of the most important properties of nanoparticles (NPs) that affects their biodistribution, accumulation and retention in the tumour tissue. The effect of NP size on tumour targeting has been studied in various contexts, with or without a targeting mechanism incorporated. Herein, we report our investigation of the size effect of NPs in Click Chemistry mediated cancer targeting. Realized by anchoring NPs onto the cell surface via metabolic glycoengineering for the cell surface placement of azido group followed by Click Chemistry mediated covalent reaction between the NP and the cell, we demonstrate that the combination effect of deeper penetration of smaller-size NP coupled with reduced clearance presumably due to the Click reaction results in its higher accumulation in the tumour tissue. Specifically, we firstly used metabolic glycoengineering to introduce azido groups to the cell surface, and then investigated the accumulation profiles of dibenzocyclooctyne conjugated nanoparticles bearing different sizes (20, 50 and 200 nm). The 20-nm silica nanoconjugates (NCs) were found to have the highest accumulation in the tumour tissue both ex vivo and in vivo. This work provides further insights into the design of nanomedicine for cancer targeting when the targeting is mediated by a non-conventional, covalent-chemistry-mediated approach.","abstract_has_math":false,"creators":["Wang, Ying"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Materials Science & Engr","degree_department":null,"school":null,"contributors":["Cheng, Jianjun"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2021,"date_issued":"2021-09-17T04:04:41Z","date_published":"2021-09-17T04:04:41Z","updated_at":"2026-07-22T22:24:52Z","subjects":["Silica Nanoparticles, Cancer targeting, metabolic engineering"],"languages":["en"],"rights":["Copyright 2021 Ying Wang"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/110842","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Cheng, Jianjun"]},{"key":"dc:creator","label":"Author","values":["Wang, Ying"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2021-09-17T04:04:41Z","2023-09-17T04:07:01Z","2021-04-28","2021-05"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Materials Science & Engr"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Silica Nanoparticles, Cancer targeting, metabolic engineering"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2021 Ying Wang"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/110842"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Size is one of the most important properties of nanoparticles (NPs) that affects their biodistribution, accumulation and retention in the tumour tissue. The effect of NP size on tumour targeting has been studied in various contexts, with or without a targeting mechanism incorporated. Herein, we report our investigation of the size effect of NPs in Click Chemistry mediated cancer targeting. Realized by anchoring NPs onto the cell surface via metabolic glycoengineering for the cell surface placement of azido group followed by Click Chemistry mediated covalent reaction between the NP and the cell, we demonstrate that the combination effect of deeper penetration of smaller-size NP coupled with reduced clearance presumably due to the Click reaction results in its higher accumulation in the tumour tissue. Specifically, we firstly used metabolic glycoengineering to introduce azido groups to the cell surface, and then investigated the accumulation profiles of dibenzocyclooctyne conjugated nanoparticles bearing different sizes (20, 50 and 200 nm). The 20-nm silica nanoconjugates (NCs) were found to have the highest accumulation in the tumour tissue both ex vivo and in vivo. This work provides further insights into the design of nanomedicine for cancer targeting when the targeting is mediated by a non-conventional, covalent-chemistry-mediated approach.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2023-05-01","The student, Ying Wang, accepted the attached license on 2021-04-23 at 13:04.","The student, Ying Wang, submitted this Thesis for approval on 2021-04-23 at 13:07.","This Thesis was approved for publication on 2021-04-28 at 15:30.","DSpace SAF Submission Ingestion Package generated from Vireo submission #16443 on 2021-09-16 at 20:11:32","Made available in DSpace on 2021-09-17T04:04:41Z (GMT). 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The effect of NP size on tumour targeting has been studied in various contexts, with or without a targeting mechanism incorporated. Herein, we report our investigation of the size effect of NPs in Click Chemistry mediated cancer targeting. Realized by anchoring NPs onto the cell surface via metabolic glycoengineering for the cell surface placement of azido group followed by Click Chemistry mediated covalent reaction between the NP and the cell, we demonstrate that the combination effect of deeper penetration of smaller-size NP coupled with reduced clearance presumably due to the Click reaction results in its higher accumulation in the tumour tissue. Specifically, we firstly used metabolic glycoengineering to introduce azido groups to the cell surface, and then investigated the accumulation profiles of dibenzocyclooctyne conjugated nanoparticles bearing different sizes (20, 50 and 200 nm). The 20-nm silica nanoconjugates (NCs) were found to have the highest accumulation in the tumour tissue both ex vivo and in vivo. This work provides further insights into the design of nanomedicine for cancer targeting when the targeting is mediated by a non-conventional, covalent-chemistry-mediated approach.","Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2023-05-01","The student, Ying Wang, accepted the attached license on 2021-04-23 at 13:04.","The student, Ying Wang, submitted this Thesis for approval on 2021-04-23 at 13:07.","This Thesis was approved for publication on 2021-04-28 at 15:30.","DSpace SAF Submission Ingestion Package generated from Vireo submission #16443 on 2021-09-16 at 20:11:32","Made available in DSpace on 2021-09-17T04:04:41Z (GMT). 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