Label-free multi-modal multiphoton imaging for real-time point-of-procedure assessment of needle biopsies

Needle biopsy (NB) is known as a standard clinical procedure for cancer diagnosis in humans and companion animals. There has been a rising concern about the time cost of the conventional histopathological assessment of the NB specimens. Therefore, a real-time point-of-procedure assessment method of NB specimens is increasingly in demand. Label-free multimodal nonlinear optical imaging (NLOI) provides a powerful and non-invasive tool to study the microstructure and biochemical distributions in biological tissue, especially in tumor microenvironments. Continuous efforts have been devoted to the development and optimization of an intraoperative label-free multimodal NLOI system that integrates four NLOI modalities: two- and three-photon fluorescence, and second and third harmonic generation. Intraoperative NLOI of fresh NB specimens from tumor-bearing animals during veterinary surgeries demonstrated the potential of this system to enable real-time and point-of-procedure histological evaluation of the NB specimens for cancer diagnosis. Further investigations have been focused on using the label-free multimodal NLOI to monitor time-lapse changes in the tissue microenvironment of the fresh NB specimens sustained in a perfusion chamber.

Name thesis:degree_name

M.S.

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Electrical & Computer Engr

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2021

Author dc:creator

• Yang, Lingxiao Lilian

Contributors dc:contributor

• Boppart, Stephen A

• Nonlinear optics
• biomedical imaging
• intraoperative imaging
• multiphoton microscopy
• cancer diagnosis

Statement dc:rights

• Copyright 2021 Lingxiao Lilian Yang

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/110676

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/110676