{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/110676"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/110676","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Label-free multi-modal multiphoton imaging for real-time point-of-procedure assessment of needle biopsies","abstract":"Needle biopsy (NB) is known as a standard clinical procedure for cancer diagnosis in humans and companion animals. There has been a rising concern about the time cost of the conventional histopathological assessment of the NB specimens. Therefore, a real-time point-of-procedure assessment method of NB specimens is increasingly in demand. Label-free multimodal nonlinear optical imaging (NLOI) provides a powerful and non-invasive tool to study the microstructure and biochemical distributions in biological tissue, especially in tumor microenvironments. Continuous efforts have been devoted to the development and optimization of an intraoperative label-free multimodal NLOI system that integrates four NLOI modalities: two- and three-photon fluorescence, and second and third harmonic generation. Intraoperative NLOI of fresh NB specimens from tumor-bearing animals during veterinary surgeries demonstrated the potential of this system to enable real-time and point-of-procedure histological evaluation of the NB specimens for cancer diagnosis. Further investigations have been focused on using the label-free multimodal NLOI to monitor time-lapse changes in the tissue microenvironment of the fresh NB specimens sustained in a perfusion chamber.","abstract_html":"Needle biopsy (NB) is known as a standard clinical procedure for cancer diagnosis in humans and companion animals. There has been a rising concern about the time cost of the conventional histopathological assessment of the NB specimens. Therefore, a real-time point-of-procedure assessment method of NB specimens is increasingly in demand. Label-free multimodal nonlinear optical imaging (NLOI) provides a powerful and non-invasive tool to study the microstructure and biochemical distributions in biological tissue, especially in tumor microenvironments. Continuous efforts have been devoted to the development and optimization of an intraoperative label-free multimodal NLOI system that integrates four NLOI modalities: two- and three-photon fluorescence, and second and third harmonic generation. Intraoperative NLOI of fresh NB specimens from tumor-bearing animals during veterinary surgeries demonstrated the potential of this system to enable real-time and point-of-procedure histological evaluation of the NB specimens for cancer diagnosis. Further investigations have been focused on using the label-free multimodal NLOI to monitor time-lapse changes in the tissue microenvironment of the fresh NB specimens sustained in a perfusion chamber.","abstract_has_math":false,"creators":["Yang, Lingxiao Lilian"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Electrical & Computer Engr","degree_department":null,"school":null,"contributors":["Boppart, Stephen A"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2021,"date_issued":"2021-09-17T02:34:31Z","date_published":"2021-09-17T02:34:31Z","updated_at":"2026-07-22T22:24:52Z","subjects":["Nonlinear optics","biomedical imaging","intraoperative imaging","multiphoton microscopy","cancer diagnosis"],"languages":["en"],"rights":["Copyright 2021 Lingxiao Lilian Yang"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/110676","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Boppart, Stephen A"]},{"key":"dc:creator","label":"Author","values":["Yang, Lingxiao Lilian"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2021-09-17T02:34:31Z","2023-09-17T02:34:57Z","2021-04-16","2021-05"]},{"key":"dc:type","label":"Dc Type","values":["text","Thesis"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Electrical & Computer Engr"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Nonlinear optics","biomedical imaging","intraoperative imaging","multiphoton microscopy","cancer diagnosis"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2021 Lingxiao Lilian Yang"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/110676"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Needle biopsy (NB) is known as a standard clinical procedure for cancer diagnosis in humans and companion animals. There has been a rising concern about the time cost of the conventional histopathological assessment of the NB specimens. Therefore, a real-time point-of-procedure assessment method of NB specimens is increasingly in demand. Label-free multimodal nonlinear optical imaging (NLOI) provides a powerful and non-invasive tool to study the microstructure and biochemical distributions in biological tissue, especially in tumor microenvironments. Continuous efforts have been devoted to the development and optimization of an intraoperative label-free multimodal NLOI system that integrates four NLOI modalities: two- and three-photon fluorescence, and second and third harmonic generation. Intraoperative NLOI of fresh NB specimens from tumor-bearing animals during veterinary surgeries demonstrated the potential of this system to enable real-time and point-of-procedure histological evaluation of the NB specimens for cancer diagnosis. Further investigations have been focused on using the label-free multimodal NLOI to monitor time-lapse changes in the tissue microenvironment of the fresh NB specimens sustained in a perfusion chamber.","Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2023-05-01","The student, Lingxiao Lilian Yang, accepted the attached license on 2021-04-15 at 11:15.","The student, Lingxiao Lilian Yang, submitted this Thesis for approval on 2021-04-15 at 11:28.","This Thesis was approved for publication on 2021-04-16 at 14:48.","DSpace SAF Submission Ingestion Package generated from Vireo submission #16346 on 2021-09-16 at 17:03:13","Made available in DSpace on 2021-09-17T02:34:31Z (GMT). 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There has been a rising concern about the time cost of the conventional histopathological assessment of the NB specimens. Therefore, a real-time point-of-procedure assessment method of NB specimens is increasingly in demand. Label-free multimodal nonlinear optical imaging (NLOI) provides a powerful and non-invasive tool to study the microstructure and biochemical distributions in biological tissue, especially in tumor microenvironments. Continuous efforts have been devoted to the development and optimization of an intraoperative label-free multimodal NLOI system that integrates four NLOI modalities: two- and three-photon fluorescence, and second and third harmonic generation. Intraoperative NLOI of fresh NB specimens from tumor-bearing animals during veterinary surgeries demonstrated the potential of this system to enable real-time and point-of-procedure histological evaluation of the NB specimens for cancer diagnosis. Further investigations have been focused on using the label-free multimodal NLOI to monitor time-lapse changes in the tissue microenvironment of the fresh NB specimens sustained in a perfusion chamber.","Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2023-05-01","The student, Lingxiao Lilian Yang, accepted the attached license on 2021-04-15 at 11:15.","The student, Lingxiao Lilian Yang, submitted this Thesis for approval on 2021-04-15 at 11:28.","This Thesis was approved for publication on 2021-04-16 at 14:48.","DSpace SAF Submission Ingestion Package generated from Vireo submission #16346 on 2021-09-16 at 17:03:13","Made available in DSpace on 2021-09-17T02:34:31Z (GMT). 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