Compressed fluorescence lifetime imaging microscope

Fluorescence lifetime imaging microscopy (FLIM) has been extensively applied in wide biomedical applications from single cell studies to medical diagnosis. While the state-of-the-art FLIM techniques are highly sensitive to biological dynamics such as fluorescence resonance emission transfer (FRET), they depend on repetitive measurements and the slow frame rate limits the study of ultrafast biological dynamics. This thesis reports the world’s fastest high-resolution FLIM at 100 frames per second (fps). This system, referred to as compressed FLIM, can capture a widefield lifetime image of 500  450 pixels within a single camera snapshot and operate real-time two-dimensional (2D) FLIM at 100 fps. By combining compressed ultrafast photography (CUP) with FLIM and adopting dual-camera detection, compressed FLIM is demonstrated for both fluorescent biological samples lifetime imaging as well as 75 fps image acquisition of fluorescent beads diffusion dynamics. Compressed FLIM holds great promise for quantitative live cell imaging applications. Compressed FLIM also has great potential in high-speed imaging of fluorescence lifetime in transient biological events such as FRET and may further extend quantitative live cell imaging such as monitoring neural spiking.

Name thesis:degree_name

M.S.

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Electrical & Computer Engr

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2019

Author dc:creator

• Ma, Yayao

Contributors dc:contributor

• Gao, Liang

• FLIM
• CUP

Statement dc:rights

• Copyright 2019 Yayao Ma

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/105865

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/105865