Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 69 for “"FLIM"”.
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Computational Dead Time Correction in Fluorescence Lifetime Imaging Microscopy (FLIM)
<p>Fluorescence Lifetime Imaging Microscopy (FLIM) is a crucial tool in biomedical research. It can visualize and measure molecular interactions and cellular processes by analyzing the fluorescence decay of molecules in biological samples. Unlike traditional imaging methods based on fluorescence …
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Fluorescence lifetime-resolved imaging microscopy studies: quantitative image analysis, spectral-FLIM, and photosynthesis
… lifetime-resolved imaging microscopy (FLIM) were carried out in this thesis. With the new instrument setup and imaging processing algorithms, valuable information has been provided for studying in vivo photosynthesis activities. Chapter 1 introduces the physics of fluorescence lifetimes …
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Optimization of fluorescence lifetime imaging microscopy (FLIM) for studying the activity of enzymes in live cancer cells
… a Fluorescence Lifetime Imaging Microscopy (FLIM) system in order to observe the dynamics of enzymes in live cancer cells. The enzyme studied throughout this research is Membrane Type 1 Matrix Metalloproteinase (MT1-MMP) which is a membrane-bound protein principally responsible for degrading …
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Compressed fluorescence lifetime imaging microscope
Fluorescence lifetime imaging microscopy (FLIM) has been extensively applied in wide biomedical applications from single cell studies to medical diagnosis. While the state-of-the-art FLIM techniques are highly sensitive to biological dynamics such as fluorescence resonance emission transfer (FRET), …
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Development & Application of Fluorescence Spectroscopy & Fluorescence Lifetime Imaging to Biology & Medicine
… lifetime-resolved imaging microscopy (FLIM). It includes work to extend the FRET and FLIM methods for studying microsecond reaction kinetics. The common thread and unifying theme of the dissertation is the development and application of fluorescence spectroscopy and microscopy (in …
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Development &Amp; Application of Fluorescence Spectroscopy &Amp; Fluorescence Lifetime Imaging to Biology &Amp; Medicine
… lifetime-resolved imaging microscopy (FLIM). It includes work to extend the FRET and FLIM methods for studying microsecond reaction kinetics. The common thread and unifying theme of the dissertation is the development and application of fluorescence spectroscopy and microscopy (in …
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LINEAR AND NONLINEAR FLUORESCENCE LIFETIME IMAGING FOR BIOSENSING APPLICATIONS
… techniques, Fluorescence Lifetime Imaging (FLIM) has gained increased importance because of their robust and versatile feature. FLIM is a technique to visualize the spatial distribution of picosecond to nanosecond excited state lifetimes within microscopic images. In this work, FLIM …
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Advancing fluorescence lifetime microscopy for quantitative bioimaging
… of fluorescence lifetime imaging microscopy (FLIM) methodologies to advance biological understanding of neurodegeneration and stem cell differentiation. Two FLIM technologies enabling faster FLIM acquisition were developed and tested for high-throughput screening of animal models of …
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Three dimensional simulation of functional neuro-vascular imaging
… physiology. Fluorescence lifetime imaging (FLIM) has been shown to allow near real time mapping of oxygen tension in plasma (pO2), and Laser Speckle Contrast Imaging (LSCI) has been demonstrated to provide qualitative assessments of blood flow in the cortex. However, as both of these methods …
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Early selection of Chinese Hamster Ovary (CHO) cells using simultaneous label-free autofluorescence multi-harmonic (slam) microscopy and Fluorescence Lifetime Imaging Microscopy (FLIM) for biopharmaceutical cell line development
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-05-01
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Modellbasierte Schätzung von Kronendeckungsgrad und -transparenz aus Landsat TM5 Fernerkundungsdaten unter Berücksichtigung reliefbedingter Beleuchtungseffekte
… sein. <br>Mit dem Forest Light Interaction Model FLIM wurde von Rosema et al (1992) ein invertierbares Bestandesreflexionsmodell entwickelt, <br>das Schattenwurf und Belaubungsdichte als steuernde Parameter des Fernerkundungssignals behandelt. In der vorliegenden Arbeit wurde <br>der Zusammenhang …
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Multi Scale Fluorescence Studies: The Spinning Disk Confocal Fluorescence Lifetime Imaging Microscopy &Amp; Single Molecule Nano-Metronome &Amp; Single Molecule Study of Tetramethyrhodamine-Dna Interaction
… domain fluorescence lifetime imaging microscopy (FLIM) and an improvement of FLIM setup to decrease the out of focus background fluorescence without drastically sacrificing the data acquisition speed while taking fluorescence lifetime data. The setup incorporates a spinning disk confocal unit to …
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Label-free multiphoton microscopy for imaging transient metabolic dynamics in living cells and tissue
… fluorescence lifetime imaging microscopy (2P-FLIM) of reduced nicotinamide adenine dinucleotide (NADH). This autofluorescent co-enzyme is involved in both aerobic and anaerobic metabolic processes which can be differentiated utilizing this advanced imaging approach. This thesis first presents …
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Investigating the mechanotransduction by two-photon fluorescence microscopy measurement of intracellular free energy of binding
… its corollary, Fluorescence Lifetime Imaging (FLIM), as well as Fluorescence Correlation Spectroscopy (FCS), was developed. A procedure by which low photon counts cell data from FLIM could be included in global analysis fits and be corrected for was developed. This results in the recovery of …
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Applying Flinet Deep Learning Model to Fluorescence Lifetime Imaging Microscopy for Lifetime Parameter Prediction
Fluorescence Lifetime Imaging Microscopy (FLIM) is essential in biomedical research, providing insights into cellular mechanisms and disease diagnosis by measuring the time molecules remain in an excited state before emitting a photon. Analyzing FLIM data is challenging due to the complex, …
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Study on gold nanoparticles for biological applications
… Fluorescence lifetime imaging microscopy (FLIM) based on two-photon luminescence lifetime from gold nanorods in cell culture, and the advantages of this method in biological imaging are demonstrated in Chapter 3. In Chapter 4, the energy transfer between a DNA dye, …
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Control of Chey-P Levels in the Chemotactic Sensory Transduction Cascade of Bacillus Subtilis
… it for new signals. The flagella proteins FliM and FliY are the interaction target for CheY-P in Bacillus subtilis. Binding of CheY-P to FliM induces a counterclockwise rotation of the motility apparatus, the flagella. FliM in Escherichia coli was previously demonstrated to have the same …
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Examining the Role of Phospholamban Phosphorylation on Interaction with SERCA Using Fluorescence Microscopy
… Fluorescence lifetime imaging microscopy (FLIM) is a variant of TR-FRET that measures fluorescence decay curves with a fast-pulsed laser and photon counting board attached to a confocal microscope. These fluorescence decay curves provide more information than intensity measurements since …
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Functional Effects of Calcium Regulation of Thin Filaments at Single Particle Resolution
… transfer based on fluorescence lifetime imaging (FLIM-FRET). Our results suggest that under physiologically relevant conditions, Tn and Tm binding to Factin is not cooperative and it is not affected by Ca2+. This suggests that one single type of interaction is involved in fully regulated thin …
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Funktionalisierte OS(T)K-Stäbe und Sensorfluorophore zur optischen Sensorik
… Zellen mittels Fluoreszenzlebenszeitmikroskopie (FLIM) untersucht. Zur Untersuchung von intrazellulärem Calcium wurden zwei verschiedene DBD-Fluorophore über einen kurzen Linker mit dem Calcium-Chelator BAPTA verknüpft. Die dargestellten Fluorophore wurden sowohl in vitro als auch in vivo auf ihre …
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