Diet-induced-obesity suppresses beta-catenin and promotes cell proliferation during colon development

Diet induced obesity (DIO), resulting from long-term consumption of a high fat diet, modifies multiple signaling pathways. These response pathways are closely associated with cell proliferation, inflammation and immune responses. The main goal of this present study is to investigate the impact from diet-induced obesity (DIO) on the regulation of expression of beta-catenin and its localization, causing downstream impacts on cellular development, inflammation and immune system in the mouse colon. In this study, 5-week-old male mice were fed either a control diet (CON, 10% kcal from fat) or a high-fat diet (HF, 45% kcal from fat) for 11 weeks. Body fat percentage and food intake results indicate significant difference between two groups of mice. The effects of DIO on mRNA expression of related genes in selected pathways in the colon tissue were analyzed in both control and DIO mice. Wnt 2, Wnt5a, Wnt7b, Axin1, APC, CTNNB1 and c-Myc were significantly decreased while Sfrp2 and Sfrp 5 were significantly increased in DIO group, suggesting that Wnt signaling pathway were suppressed by DIO. The expression of p21 and Raf-1 were also significantly decreased in DIO group, while Cyclin D1 was increased, suggesting that DIO has impact on cell proliferation. Immunofluorescent staining results suggested that repressed Wnt signaling prevented beta-catenin translocation and its nuclear accumulation, while Ki-67 showed a stronger signal intense in DIO mice. Overall, this study shows that DIO suppressed beta-catenin expression as well as nuclear accumulation, which inhibited Wnt signaling pathway while promoted cell proliferation in the colon of DIO mouse. Furthermore, findings from current study may be applied as colon health indicator for clinical study in future.

Name thesis:degree_name

M.S.

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Food Science & Human Nutrition

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2018

Author dc:creator

• Xu, Guanying

Contributors dc:contributor

• Pan, Yuan-Xiang
• Chen, Hong
• Arthur, Anna E

• diet-induced-obesity
• wnt
• beta-catenin
• proliferation

Statement dc:rights

• Copyright 2018 Guanying Xu

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/101719

OAI record oai:identifier

https://www.ideals.illinois.edu/oai-pmh?verb=GetRecord&metadataPrefix=etdms&identifier=oai%3Awww.ideals.illinois.edu%3A2142%2F101719