{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/101719"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/101719","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Diet-induced-obesity suppresses beta-catenin and promotes cell proliferation during colon development","abstract":"The student, Guanying Xu, submitted this Thesis for approval on 2018-07-17 at 11:44.","abstract_html":"The student, Guanying Xu, submitted this Thesis for approval on 2018-07-17 at 11:44.","abstract_has_math":false,"creators":["Xu, Guanying"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Food Science & Human Nutrition","degree_department":null,"school":null,"contributors":["Pan, Yuan-Xiang","Chen, Hong","Arthur, Anna E"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2018,"date_issued":"2018-09-27T16:34:21Z","date_published":"2018-09-27T16:34:21Z","updated_at":"2026-07-22T22:24:40Z","subjects":["diet-induced-obesity","wnt","beta-catenin","proliferation"],"languages":["en"],"rights":["Copyright 2018 Guanying Xu"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/101719","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Pan, Yuan-Xiang","Chen, Hong","Arthur, Anna E"]},{"key":"dc:creator","label":"Author","values":["Xu, Guanying"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2018-09-27T16:34:21Z","2020-09-28T09:15:16Z","2018-07-19","2018-08"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Food Science & Human Nutrition"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["diet-induced-obesity","wnt","beta-catenin","proliferation"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]},{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2018 Guanying Xu"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/101719"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The student, Guanying Xu, submitted this Thesis for approval on 2018-07-17 at 11:44.","This Thesis was approved for publication on 2018-07-19 at 08:26.","DSpace SAF Submission Ingestion Package generated from Vireo submission #12886 on 2018-09-27 at 11:19:21","Diet induced obesity (DIO), resulting from long-term consumption of a high fat diet, modifies multiple signaling pathways. These response pathways are closely associated with cell proliferation, inflammation and immune responses. The main goal of this present study is to investigate the impact from diet-induced obesity (DIO) on the regulation of expression of beta-catenin and its localization, causing downstream impacts on cellular development, inflammation and immune system in the mouse colon. In this study, 5-week-old male mice were fed either a control diet (CON, 10% kcal from fat) or a high-fat diet (HF, 45% kcal from fat) for 11 weeks. Body fat percentage and food intake results indicate significant difference between two groups of mice. The effects of DIO on mRNA expression of related genes in selected pathways in the colon tissue were analyzed in both control and DIO mice. Wnt 2, Wnt5a, Wnt7b, Axin1, APC, CTNNB1 and c-Myc were significantly decreased while Sfrp2 and Sfrp 5 were significantly increased in DIO group, suggesting that Wnt signaling pathway were suppressed by DIO. The expression of p21 and Raf-1 were also significantly decreased in DIO group, while Cyclin D1 was increased, suggesting that DIO has impact on cell proliferation. Immunofluorescent staining results suggested that repressed Wnt signaling prevented beta-catenin translocation and its nuclear accumulation, while Ki-67 showed a stronger signal intense in DIO mice. Overall, this study shows that DIO suppressed beta-catenin expression as well as nuclear accumulation, which inhibited Wnt signaling pathway while promoted cell proliferation in the colon of DIO mouse. Furthermore, findings from current study may be applied as colon health indicator for clinical study in future.","Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2020-08-01","The student, Guanying Xu, accepted the attached license on 2018-07-17 at 11:42.","Made available in DSpace on 2018-09-27T16:34:21Z (GMT). No. of bitstreams: 2 XU-THESIS-2018.pdf: 1170506 bytes, checksum: f6b45f243b5ec4d38462ec7768ee3f94 (MD5) LICENSE.txt: 4206 bytes, checksum: 29268d9089eb3422c5feabd35ccad9f3 (MD5) Previous issue date: 2018-07-19","Embargo set by: Seth Robbins for item 107819 Lift date: 2020-09-27T16:34:29Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system","U of I Only Restriction Lifted for Item 107819 on 2020-09-28T09:15:16Z."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Diet-induced-obesity suppresses beta-catenin and promotes cell proliferation during colon development"]}]}],"canonical_facts":{"dc:contributor":["Pan, Yuan-Xiang","Chen, Hong","Arthur, Anna E"],"dc:creator":["Xu, Guanying"],"dc:date":["2018-09-27T16:34:21Z","2020-09-28T09:15:16Z","2018-07-19","2018-08"],"dc:description":["The student, Guanying Xu, submitted this Thesis for approval on 2018-07-17 at 11:44.","This Thesis was approved for publication on 2018-07-19 at 08:26.","DSpace SAF Submission Ingestion Package generated from Vireo submission #12886 on 2018-09-27 at 11:19:21","Diet induced obesity (DIO), resulting from long-term consumption of a high fat diet, modifies multiple signaling pathways. These response pathways are closely associated with cell proliferation, inflammation and immune responses. The main goal of this present study is to investigate the impact from diet-induced obesity (DIO) on the regulation of expression of beta-catenin and its localization, causing downstream impacts on cellular development, inflammation and immune system in the mouse colon. In this study, 5-week-old male mice were fed either a control diet (CON, 10% kcal from fat) or a high-fat diet (HF, 45% kcal from fat) for 11 weeks. Body fat percentage and food intake results indicate significant difference between two groups of mice. The effects of DIO on mRNA expression of related genes in selected pathways in the colon tissue were analyzed in both control and DIO mice. Wnt 2, Wnt5a, Wnt7b, Axin1, APC, CTNNB1 and c-Myc were significantly decreased while Sfrp2 and Sfrp 5 were significantly increased in DIO group, suggesting that Wnt signaling pathway were suppressed by DIO. The expression of p21 and Raf-1 were also significantly decreased in DIO group, while Cyclin D1 was increased, suggesting that DIO has impact on cell proliferation. Immunofluorescent staining results suggested that repressed Wnt signaling prevented beta-catenin translocation and its nuclear accumulation, while Ki-67 showed a stronger signal intense in DIO mice. Overall, this study shows that DIO suppressed beta-catenin expression as well as nuclear accumulation, which inhibited Wnt signaling pathway while promoted cell proliferation in the colon of DIO mouse. Furthermore, findings from current study may be applied as colon health indicator for clinical study in future.","Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2020-08-01","The student, Guanying Xu, accepted the attached license on 2018-07-17 at 11:42.","Made available in DSpace on 2018-09-27T16:34:21Z (GMT). No. of bitstreams: 2 XU-THESIS-2018.pdf: 1170506 bytes, checksum: f6b45f243b5ec4d38462ec7768ee3f94 (MD5) LICENSE.txt: 4206 bytes, checksum: 29268d9089eb3422c5feabd35ccad9f3 (MD5) Previous issue date: 2018-07-19","Embargo set by: Seth Robbins for item 107819 Lift date: 2020-09-27T16:34:29Z Reason: Author requested U of Illinois access only (OA after 2yrs) in Vireo ETD system","U of I Only Restriction Lifted for Item 107819 on 2020-09-28T09:15:16Z."],"dc:format":["application/pdf"],"dc:identifier":["http://hdl.handle.net/2142/101719"],"dc:language":["en"],"dc:rights":["Copyright 2018 Guanying Xu"],"dc:subject":["diet-induced-obesity","wnt","beta-catenin","proliferation"],"dc:title":["Diet-induced-obesity suppresses beta-catenin and promotes cell proliferation during colon development"],"dc:type":["text"],"thesis:degree_discipline":["Food Science & Human Nutrition"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["M.S."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:24:40Z"}