Molecular characterization and nucleotide sequence of the coat protein gene of the NL-3 strain of bean common mosaic virus

Bean common mosaic virus (BCMV) is a member of the potyvirus group, causing severe damage to leguminous crops. The NL-3 strain of the virus was purified and viral RNA extracted. Complementary DNA was synthesized from the viral RNA using Moloney-murine leukemia reverse transcriptase and Xho I oligo-dT priming. The single-stranded cDNA was converted into ds DNA by DNA polymerase and directionally cloned into lambda Uni-ZAP XR vector. The coat protein gene of the potyvirus is located at the extreme 3$\sp\prime$ end of the viral RNA, so clones of approximately 1 kb were selected, subcloned, and sequenced by the dideoxy chain termination method. Results from the nucleotide sequencing indicate that areas of homology and non-homology exist between the NL-3 strain and other published potyviral coat protein sequence data.

Name thesis:degree_name

Doctor of Philosophy

Level thesis:degree_level

Doctoral

Discipline thesis:degree_discipline

Molecular Biology

Grantor

Texas Woman's University

Year dc:date.issued

1993

Author dc:creator

• Sparks, Mary Jean

Chair dc:contributor.committeechair

• Knesek, John

Committee members dc:contributor.committeemember

• Mills, Nathaniel
• Cockerline, Alan
• Rudick, Michael

• Bean Commen Mosaic Virus
• Nucleotide sequence

Language dc:language.iso

en_US

Handle dc:identifier.uri

https://hdl.handle.net/11274/14111

OAI identifier oai:identifier

oai:twu-ir.tdl.org:11274/14111