{"id":{"repo_id":"twu","oai_identifier":"oai:twu-ir.tdl.org:11274/14111"},"canonical_url":"https://search.dev.ndltd.org/etd/twu/oai:twu-ir.tdl.org:11274/14111","repository":{"repo_id":"twu","name":"Texas Woman's University","base_url":"https://twu-ir.tdl.org/server/oai/request"},"display":{"title":"Molecular characterization and nucleotide sequence of the coat protein gene of the NL-3 strain of bean common mosaic virus","abstract":"Bean common mosaic virus (BCMV) is a member of the potyvirus group, causing severe damage to leguminous crops. The NL-3 strain of the virus was purified and viral RNA extracted. Complementary DNA was synthesized from the viral RNA using Moloney-murine leukemia reverse transcriptase and Xho I oligo-dT priming. The single-stranded cDNA was converted into ds DNA by DNA polymerase and directionally cloned into lambda Uni-ZAP XR vector. The coat protein gene of the potyvirus is located at the extreme 3$\\sp\\prime$ end of the viral RNA, so clones of approximately 1 kb were selected, subcloned, and sequenced by the dideoxy chain termination method. Results from the nucleotide sequencing indicate that areas of homology and non-homology exist between the NL-3 strain and other published potyviral coat protein sequence data.","abstract_html":"Bean common mosaic virus (BCMV) is a member of the potyvirus group, causing severe damage to leguminous crops. The NL-3 strain of the virus was purified and viral RNA extracted. Complementary DNA was synthesized from the viral RNA using Moloney-murine leukemia reverse transcriptase and Xho I oligo-dT priming. The single-stranded cDNA was converted into ds DNA by DNA polymerase and directionally cloned into lambda Uni-ZAP XR vector. The coat protein gene of the potyvirus is located at the extreme 3$\\sp\\prime$ end of the viral RNA, so clones of approximately 1 kb were selected, subcloned, and sequenced by the dideoxy chain termination method. Results from the nucleotide sequencing indicate that areas of homology and non-homology exist between the NL-3 strain and other published potyviral coat protein sequence data.","abstract_has_math":true,"creators":["Sparks, Mary Jean"],"institution":"Texas Woman's University","degree_name":"Doctor of Philosophy","degree_level":"Doctoral","degree_discipline":"Molecular Biology","degree_department":null,"school":null,"contributors":[],"advisors":[],"committee_chairs":["Knesek, John"],"committee_members":["Mills, Nathaniel","Cockerline, Alan","Rudick, Michael"],"year":1993,"date_issued":"1993-05","date_published":"1993-05","updated_at":"2026-07-24T05:04:55Z","subjects":["Bean Commen Mosaic Virus","Nucleotide sequence"],"languages":["en_US"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://hdl.handle.net/11274/14111","outbound_label":"Handle","outbound_source":"dc:identifier.uri"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor.committeechair","label":"Committee Chair","values":["Knesek, John"]},{"key":"dc:contributor.committeemember","label":"Committee Member","values":["Mills, Nathaniel","Cockerline, Alan","Rudick, Michael"]},{"key":"dc:creator","label":"Author","values":["Sparks, Mary Jean"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.accessioned","label":"Dc Date Accessioned","values":["2022-10-19T17:56:31Z"]},{"key":"dc:date.available","label":"Dc Date Available","values":["2022-10-19T17:56:31Z"]},{"key":"dc:date.issued","label":"Date","values":["1993-05"]},{"key":"dc:type","label":"Dc Type","values":["Dissertation"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Molecular Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Doctoral"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Doctor of Philosophy"]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["Texas Woman's University"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Bean Commen Mosaic Virus","Nucleotide sequence"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language.iso","label":"Language (ISO)","values":["en_US"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier.uri","label":"Identifier URI","values":["https://hdl.handle.net/11274/14111"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Bean common mosaic virus (BCMV) is a member of the potyvirus group, causing severe damage to leguminous crops. The NL-3 strain of the virus was purified and viral RNA extracted. Complementary DNA was synthesized from the viral RNA using Moloney-murine leukemia reverse transcriptase and Xho I oligo-dT priming. The single-stranded cDNA was converted into ds DNA by DNA polymerase and directionally cloned into lambda Uni-ZAP XR vector. The coat protein gene of the potyvirus is located at the extreme 3$\\sp\\prime$ end of the viral RNA, so clones of approximately 1 kb were selected, subcloned, and sequenced by the dideoxy chain termination method. Results from the nucleotide sequencing indicate that areas of homology and non-homology exist between the NL-3 strain and other published potyviral coat protein sequence data."]},{"key":"dc:title","label":"Title","values":["Molecular characterization and nucleotide sequence of the coat protein gene of the NL-3 strain of bean common mosaic virus"]}]}],"canonical_facts":{"dc:contributor.committeechair":["Knesek, John"],"dc:contributor.committeemember":["Mills, Nathaniel","Cockerline, Alan","Rudick, Michael"],"dc:creator":["Sparks, Mary Jean"],"dc:date.accessioned":["2022-10-19T17:56:31Z"],"dc:date.available":["2022-10-19T17:56:31Z"],"dc:date.issued":["1993-05"],"dc:description.abstract":["Bean common mosaic virus (BCMV) is a member of the potyvirus group, causing severe damage to leguminous crops. The NL-3 strain of the virus was purified and viral RNA extracted. Complementary DNA was synthesized from the viral RNA using Moloney-murine leukemia reverse transcriptase and Xho I oligo-dT priming. The single-stranded cDNA was converted into ds DNA by DNA polymerase and directionally cloned into lambda Uni-ZAP XR vector. The coat protein gene of the potyvirus is located at the extreme 3$\\sp\\prime$ end of the viral RNA, so clones of approximately 1 kb were selected, subcloned, and sequenced by the dideoxy chain termination method. Results from the nucleotide sequencing indicate that areas of homology and non-homology exist between the NL-3 strain and other published potyviral coat protein sequence data."],"dc:identifier.uri":["https://hdl.handle.net/11274/14111"],"dc:language.iso":["en_US"],"dc:subject":["Bean Commen Mosaic Virus","Nucleotide sequence"],"dc:title":["Molecular characterization and nucleotide sequence of the coat protein gene of the NL-3 strain of bean common mosaic virus"],"dc:type":["Dissertation"],"thesis:degree_discipline":["Molecular Biology"],"thesis:degree_level":["Doctoral"],"thesis:degree_name":["Doctor of Philosophy"],"thesis:institution_name":["Texas Woman's University"]},"updated_at":"2026-07-24T05:04:55Z"}