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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 155 for “"Nucleotide sequence"”.
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Characterization and nucleotide sequence of apple mosaic virus coat protein gene
… clones from the 3$\sp\prime$-end of RNA3 were sequenced and the 3$\sp\prime$-primer was designed to synthesize cDNA of RNA3. The DNA was cloned into the plasmid vector pUC118. A set of overlapping cDNA clones was used to determine the nucleotide sequence of RNA4. The 5$\sp\prime$-end of RNA4 …
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The nucleotide sequence of the 3' terminus of soybean mosaic virus
The nucleotide sequence of the 3' terminus of soybean mosaic virus (SMV) VA/G1 RNA has been determined by dideoxynucleotide sequencing of oligo(dT) primed cDNA cloned into a pUC19 cloning vector via EcoR1 linkers. One recombinant plasmid, pSMV49, identified by colony and dot-blot hybridization to …
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Molecular characterization and nucleotide sequence of the coat protein gene of the NL-3 strain of bean common mosaic virus
… approximately 1 kb were selected, subcloned, and sequenced by the dideoxy chain termination method. Results from the nucleotide sequencing indicate that areas of homology and non-homology exist between the NL-3 strain and other published potyviral coat protein sequence data.
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Thelin Operon in Pseudomonas Incognita: Cloning, Nucleotide Sequence and Deduced Amino Acid Sequence of Cytochrome P-450lin and Other Enzymes Involved in the Catabolism of Linalool
… and tryptic-fragment amino acid sequence, the cytochrome P-450lin operon (lin) was cloned. Four overlapping clones representing 10.8 kb of novel sequence were isolated and sequenced. Eight open reading frames were found in this sequence. The GenBank, EMBL, and SwissProt databases …
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Characterization of the <i>glpD</i> and <i>glpEGR</i> operons of <i>Escherichia coli</i> k-12
… membrane-associated respiratory enzyme. The nucleotide sequence of <i>glpD</i> was determined. An open reading frame of 501 codons was preceded by a consensus Shine-Dalgarno sequence. The proposed translational start and reading frame of glpD were confirmed by determining the nucleotide …
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Structure and function of the repressor and operators of the sn-glycerol-3-phosphate regulon of Escherichia coli K-12
… Escherichia coli K-12. In the present study, the nucleotide sequence of the 2895 base pair of DNA containing the glpD control region and the glpE, glpG, glpR genes was determined. The translation initiation codons with adjacent ribosome-binding sites were found for these four genes. The …
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Strain differentiation and screening for resistance against isolates of soybean mosaic virus
… the SMV G1 strain. The techniques were based on nucleotide sequence differences in that part of the SMV genome that spans the mid-region of the coat protein gene to the end of the non-coding region (NCR), and in the CI protein-coding region of the genome.
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Sturcture and function of tomato ringspot virus RNA1 and RNA2
… genome were obtained and used to determine the nucleotide sequence of RNA1and RNA2. The 5' termini of RNA1 and RNA2, which were not present in any cDNA clone analyzed, were determined by using viral RNA as a template in dideoxynucleotide sequencing reactions. TomRSV RNA1 is 8,214 nucleotides …
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Betaine-Homocysteine Methyltransferase: Tissue Distribution, Nutrient Regulation, Genomic Organization, and Promoter Isolation
The electrophoretic mobility (45 kDa) and the nucleotide sequence of the human kidney BHMT has been shown to be identical to liver BHMT. Initial rate data was plotted according to the method of Hanes and the Michaelis Constants were estimated to be 2160 muM and <10 muM for betaine and …
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The Mechanism of Cap-Independent Initiation of Translation of Satellite Tobacco Necrosis Virus RNA
… region of STNV RNA contains a nucleotide sequence that is complementary to the 3$\sp\prime$-terminal region of the 18S rRNA.
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Molecular analysis of the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli
… and E. coli(pVS100) is regulated. The nucleotide sequence and analysis of DNA regions encoding the sucrose uptake and regulatory functions are presented here. An investigation of the expression of the. V. alginolyticus sucrose utilization system in Bacillus subtilis is also presented.
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Genetic analysis of nifF and nifA and site-directed mutagenesis of nifE in Azotobacter vinelandii
… vinelandii</i> were genetically analyzed. The nucleotide sequence of the nifF gene, which encodes a flavodoxin, was determined. Specific mutation strains indicated that in <i>A vinelandii</i> flavodoxin is not the unique physiological electron donor to nitrogenase. The nifF gene appears to be …
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Structural Analysis of the TOL pDK1 xylGFJQK Region and Partial Characterization of the xylF and xylG Gene Products
… them to central metabolic intermediates. The nucleotide sequence for the 5.6 kb xylGFJQK region of the pDK1 TOL meta operon was determined. DNA sequence analysis revealed the presence of five open reading frames corresponding to xylG (1458 bp), xylF (846 bp), xylJ (783 bp), xylQ (936 bp) and …
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Analysis of catabolic plasmids in pseudomonas
… vectors and analysed by DNA sequencing. The nucleotide sequence of the 5' end portion of the C230 gene comprising 231 nucleotides was determined. Taken together with previous nucleotide sequence data, the first 445 nucleotides of the gene, and its upstream region, are presented here. …
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CBK Bacteriophage Genome Annotation and Organization
… being the best studied. As no B3 phage genome sequences are currently available in the Genbank, we decided to determine the nucleotide sequence of the CBK genome. Bacteriophage CbK is a double-stranded DNA phage with an extremely large genome size of 203,242 base pairs and a 66.13% GC content. …
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Sequence, Expression, and Mutagenesis of the Pseudomonas Putida Cytochrome P450(cam) Gene in Escherichia Coli
… This investigation reports the complete nucleotide sequence of the camC gene along with 155 base pairs of 5$\sp\prime$ and approximately 790 base pairs of 3$\sp\prime$ flanking sequence. The gene encoding putidaredoxin reductase, camA, was located 22 nucleotides downstream from the camC …
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Molecular characterization of the major envelope protein of porcine reproductive and respiratory syndrome virus (PRRSV) and evaluation of its use for a diagnostic assay, vaccine development, and the examination of quasispecies evolution
… (GP5) of acute PRRSV isolates was characterized. Sequence and phylogenetic analyses revealed that seven of the acute PRRS virus (PRRSV) isolates were related to other N. American PRRSV isolates while one isolate, 98-37120-2, was very closely related to and may have been derived from the MLV, …
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