Arachidin 3 Modulation of Lipid Metabolism in Rotavirus Infections

<p>Rotavirus (RV) can cause severe and deadly gastroenteritis in young children, infants, and immunocompromised individuals. Previous studies have shown that arachidin 3 (A3) inhibits RV replication, and that RV replication is dependent on the presence of lipids. This study investigated the alteration of lipid metabolism by A3 in RV infected HT29.f8 cells. A decrease in the RV regulation of lipid biosynthesis genes was observed with the addition of A3 using qRT-PCR. Also, immunofluorescent and histochemical staining for neutral fats, a major component of cellular lipid droplets, revealed an increased accumulation with both RV and RV+A3 when compared to no virus and A3 controls. Furthermore, a western blot time course study of perilipin 1 presented a cycling pattern of expression with slight variations between RV, RV+A3, and A3. This data implies an association between A3 inhibition of RV replication and lipid metabolism that could be developed into a RV therapeutic treatment.</p>

Name thesis:degree_name

Master of Science - Biotechnology

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Biology

Year dc:date.available

2019

Author dc:creator

• Wisdom, Stormey

Contributors dc:contributor

• Rebecca D. Parr, Ph.D
• Odutayo Odunuga, Ph.D
• Josephine Taylor, Ph.D

• Virology
• HGMGCR
• LDLR
• lipidology
• Biochemistry
• Carbohydrates
• Digestive System Diseases
• Lipids
• Molecular Biology
• Other Immunology and Infectious Disease
• Virus Diseases

Repository record dc:identifier

https://scholarworks.sfasu.edu/etds/243

OAI identifier oai:identifier

oai:scholarworks.sfasu.edu:etds-1305