Rotavirus (RV) can cause severe and deadly gastroenteritis in young children, infants, and immunocompromised individuals. Previous studies have shown that arachidin 3 (A3) inhibits RV replication, and that RV replication is dependent on the presence of lipids. This study investigated the alteration of lipid metabolism by A3 in RV infected HT29.f8 cells. A decrease in the RV regulation of lipid biosynthesis genes was observed with the addition of A3 using qRT-PCR. Also, immunofluorescent and histochemical staining for neutral fats, a major component of cellular lipid droplets, revealed an increased accumulation with both RV and RV+A3 when compared to no virus and A3 controls. Furthermore, a western blot time course study of perilipin 1 presented a cycling pattern of expression with slight variations between RV, RV+A3, and A3. This data implies an association between A3 inhibition of RV replication and lipid metabolism that could be developed into a RV therapeutic treatment.
","abstract_html":"<p>Rotavirus (RV) can cause severe and deadly gastroenteritis in young children, infants, and immunocompromised individuals. Previous studies have shown that arachidin 3 (A3) inhibits RV replication, and that RV replication is dependent on the presence of lipids. This study investigated the alteration of lipid metabolism by A3 in RV infected HT29.f8 cells. A decrease in the RV regulation of lipid biosynthesis genes was observed with the addition of A3 using qRT-PCR. Also, immunofluorescent and histochemical staining for neutral fats, a major component of cellular lipid droplets, revealed an increased accumulation with both RV and RV+A3 when compared to no virus and A3 controls. Furthermore, a western blot time course study of perilipin 1 presented a cycling pattern of expression with slight variations between RV, RV+A3, and A3. This data implies an association between A3 inhibition of RV replication and lipid metabolism that could be developed into a RV therapeutic treatment.</p>","abstract_has_math":false,"creators":["Wisdom, Stormey"],"institution":null,"degree_name":"Master of Science - Biotechnology","degree_level":"Thesis","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Rebecca D. Parr, Ph.D","Odutayo Odunuga, Ph.D","Josephine Taylor, Ph.D"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2019,"date_issued":"2019-06-01T07:00:00Z","date_published":"2019-06-01T07:00:00Z","updated_at":"2026-07-24T04:30:22Z","subjects":["Virology","HGMGCR","LDLR","lipidology","Biochemistry","Carbohydrates","Digestive System Diseases","Lipids","Molecular Biology","Other Immunology and Infectious Disease","Virus Diseases"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://scholarworks.sfasu.edu/etds/243","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Rebecca D. Parr, Ph.D","Odutayo Odunuga, Ph.D","Josephine Taylor, Ph.D"]},{"key":"dc:creator","label":"Author","values":["Wisdom, Stormey"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2021-08-20T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science - Biotechnology"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Virology","HGMGCR","LDLR","lipidology","Biochemistry","Carbohydrates","Digestive System Diseases","Lipids","Molecular Biology","Other Immunology and Infectious Disease","Virus Diseases"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarworks.sfasu.edu/etds/243"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Rotavirus (RV) can cause severe and deadly gastroenteritis in young children, infants, and immunocompromised individuals. Previous studies have shown that arachidin 3 (A3) inhibits RV replication, and that RV replication is dependent on the presence of lipids. This study investigated the alteration of lipid metabolism by A3 in RV infected HT29.f8 cells. A decrease in the RV regulation of lipid biosynthesis genes was observed with the addition of A3 using qRT-PCR. Also, immunofluorescent and histochemical staining for neutral fats, a major component of cellular lipid droplets, revealed an increased accumulation with both RV and RV+A3 when compared to no virus and A3 controls. Furthermore, a western blot time course study of perilipin 1 presented a cycling pattern of expression with slight variations between RV, RV+A3, and A3. This data implies an association between A3 inhibition of RV replication and lipid metabolism that could be developed into a RV therapeutic treatment.
"]},{"key":"dc:title","label":"Title","values":["Arachidin 3 Modulation of Lipid Metabolism in Rotavirus Infections"]}]}],"canonical_facts":{"dc:contributor":["Rebecca D. Parr, Ph.D","Odutayo Odunuga, Ph.D","Josephine Taylor, Ph.D"],"dc:creator":["Wisdom, Stormey"],"dc:date.available":["2021-08-20T07:00:00Z"],"dc:description.abstract":["Rotavirus (RV) can cause severe and deadly gastroenteritis in young children, infants, and immunocompromised individuals. Previous studies have shown that arachidin 3 (A3) inhibits RV replication, and that RV replication is dependent on the presence of lipids. This study investigated the alteration of lipid metabolism by A3 in RV infected HT29.f8 cells. A decrease in the RV regulation of lipid biosynthesis genes was observed with the addition of A3 using qRT-PCR. Also, immunofluorescent and histochemical staining for neutral fats, a major component of cellular lipid droplets, revealed an increased accumulation with both RV and RV+A3 when compared to no virus and A3 controls. Furthermore, a western blot time course study of perilipin 1 presented a cycling pattern of expression with slight variations between RV, RV+A3, and A3. This data implies an association between A3 inhibition of RV replication and lipid metabolism that could be developed into a RV therapeutic treatment.
"],"dc:identifier":["https://scholarworks.sfasu.edu/etds/243"],"dc:subject":["Virology","HGMGCR","LDLR","lipidology","Biochemistry","Carbohydrates","Digestive System Diseases","Lipids","Molecular Biology","Other Immunology and Infectious Disease","Virus Diseases"],"dc:title":["Arachidin 3 Modulation of Lipid Metabolism in Rotavirus Infections"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science - Biotechnology"]},"updated_at":"2026-07-24T04:30:22Z"}