Enzymatic Sulfurylation Mechanisms

<p>Sulfate activation and transfer to lipids, polysaccharides, and aminoalkyl phenols have been studied In a variety of biological systems. The transfer of S³⁵O₄ from radioactive 3'-phosphoadenoslne 5'-phosphosulfate (PAPS³⁵) into lipid-soluble material has been investigated in cell-free preparations of rat brain and liver. In brain of 10-20 day old rats activity was found in the particulate fraction of the homogenate, whereas in young adult rat liver activity resided in the supernatant fraction (105,000 x g). In both systems incorporation was stimulated by the addition of a crude mixture of the isomers of N-acetyl sphingosine. Evidence accumulated suggesting that the radioactive sulfolipids produced by these two system were probably different.</p>

Name thesis:degree_name

Doctor of Philosophy (PhD)

Level thesis:degree_level

Thesis

Year

1960

Author dc:creator

• Goldberg, Irving Hyman

Contributors dc:contributor

• Fritz Lipmann

• sulfate activation
• PAPS
• sulfolipids
• rat brain
• rat liver
• N-acetyl sphingosine
• Life Sciences

Repository record dc:identifier

https://digitalcommons.rockefeller.edu/student_theses_and_dissertations/581

OAI identifier oai:identifier

oai:digitalcommons.rockefeller.edu:student_theses_and_dissertations-1584