Sulfate activation and transfer to lipids, polysaccharides, and aminoalkyl phenols have been studied In a variety of biological systems. The transfer of S35O4 from radioactive 3'-phosphoadenoslne 5'-phosphosulfate (PAPS35) into lipid-soluble material has been investigated in cell-free preparations of rat brain and liver. In brain of 10-20 day old rats activity was found in the particulate fraction of the homogenate, whereas in young adult rat liver activity resided in the supernatant fraction (105,000 x g). In both systems incorporation was stimulated by the addition of a crude mixture of the isomers of N-acetyl sphingosine. Evidence accumulated suggesting that the radioactive sulfolipids produced by these two system were probably different.
","abstract_html":"<p>Sulfate activation and transfer to lipids, polysaccharides, and aminoalkyl phenols have been studied In a variety of biological systems. The transfer of S<sup>35</sup>O<sub>4</sub> from radioactive 3'-phosphoadenoslne 5'-phosphosulfate (PAPS<sup>35</sup>) into lipid-soluble material has been investigated in cell-free preparations of rat brain and liver. In brain of 10-20 day old rats activity was found in the particulate fraction of the homogenate, whereas in young adult rat liver activity resided in the supernatant fraction (105,000 x g). In both systems incorporation was stimulated by the addition of a crude mixture of the isomers of N-acetyl sphingosine. Evidence accumulated suggesting that the radioactive sulfolipids produced by these two system were probably different.</p>","abstract_has_math":false,"creators":["Goldberg, Irving Hyman"],"institution":null,"degree_name":"Doctor of Philosophy (PhD)","degree_level":"Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":["Fritz Lipmann"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1960,"date_issued":"1960-01-01T08:00:00Z","date_published":"1960-01-01T08:00:00Z","updated_at":"2026-07-24T04:11:28Z","subjects":["sulfate activation","PAPS","sulfolipids","rat brain","rat liver","N-acetyl sphingosine","Life Sciences"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://digitalcommons.rockefeller.edu/student_theses_and_dissertations/581","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Fritz Lipmann"]},{"key":"dc:creator","label":"Author","values":["Goldberg, Irving Hyman"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Doctor of Philosophy (PhD)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["sulfate activation","PAPS","sulfolipids","rat brain","rat liver","N-acetyl sphingosine","Life Sciences"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://digitalcommons.rockefeller.edu/student_theses_and_dissertations/581"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Sulfate activation and transfer to lipids, polysaccharides, and aminoalkyl phenols have been studied In a variety of biological systems. The transfer of S35O4 from radioactive 3'-phosphoadenoslne 5'-phosphosulfate (PAPS35) into lipid-soluble material has been investigated in cell-free preparations of rat brain and liver. In brain of 10-20 day old rats activity was found in the particulate fraction of the homogenate, whereas in young adult rat liver activity resided in the supernatant fraction (105,000 x g). In both systems incorporation was stimulated by the addition of a crude mixture of the isomers of N-acetyl sphingosine. Evidence accumulated suggesting that the radioactive sulfolipids produced by these two system were probably different.
"]},{"key":"dc:title","label":"Title","values":["Enzymatic Sulfurylation Mechanisms"]}]}],"canonical_facts":{"dc:contributor":["Fritz Lipmann"],"dc:creator":["Goldberg, Irving Hyman"],"dc:description.abstract":["Sulfate activation and transfer to lipids, polysaccharides, and aminoalkyl phenols have been studied In a variety of biological systems. The transfer of S35O4 from radioactive 3'-phosphoadenoslne 5'-phosphosulfate (PAPS35) into lipid-soluble material has been investigated in cell-free preparations of rat brain and liver. In brain of 10-20 day old rats activity was found in the particulate fraction of the homogenate, whereas in young adult rat liver activity resided in the supernatant fraction (105,000 x g). In both systems incorporation was stimulated by the addition of a crude mixture of the isomers of N-acetyl sphingosine. Evidence accumulated suggesting that the radioactive sulfolipids produced by these two system were probably different.
"],"dc:identifier":["https://digitalcommons.rockefeller.edu/student_theses_and_dissertations/581"],"dc:subject":["sulfate activation","PAPS","sulfolipids","rat brain","rat liver","N-acetyl sphingosine","Life Sciences"],"dc:title":["Enzymatic Sulfurylation Mechanisms"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Doctor of Philosophy (PhD)"]},"updated_at":"2026-07-24T04:11:28Z"}