Induction of Innate and Adaptive Immunity In Vivo by Selective Antibody Targeting of Poly dA:dT and Antigen to Dendritic Cells

<p>Dendritic cells (DCs) have been shown to be efficient and specialized in antigen presentation, but innate stimuli have not been selectively delivered to determine if these cells alone can integrate innate and adaptive immunity in vivo. Here we illustrate a novel method using expressed protein ligation (EPL) to attach the immune stimulant, poly dA:dT, to the DC uptake receptor, DEC205 (DEC). We will show that inoculation of α-DEC-poly dA:dT fusion monoclonal antibodies (mAbs) is sufficient to induce rapid innate cytokine production by DCs. Adaptive T cell immunity is also elicited when the fusion mAb is given in combination with a DC-targeted antigen. Forced entry is not required because α-DEC fusion mAbs deliver the poly dA:dT to its cytosolic targets.</p>

Name thesis:degree_name

Doctor of Philosophy (PhD)

Level thesis:degree_level

Thesis

Year

2012

Author dc:creator

• Barbuto, Scott A

Contributors dc:contributor

• Ralph Steinman

• dendritic cells
• antigen presentation
• poly dA:dT
• DEC205
• innate immunity
• adaptive immunity
• Life Sciences

Repository record dc:identifier

https://digitalcommons.rockefeller.edu/student_theses_and_dissertations/243

OAI identifier oai:identifier

oai:digitalcommons.rockefeller.edu:student_theses_and_dissertations-1241