Dendritic cells (DCs) have been shown to be efficient and specialized in antigen presentation, but innate stimuli have not been selectively delivered to determine if these cells alone can integrate innate and adaptive immunity in vivo. Here we illustrate a novel method using expressed protein ligation (EPL) to attach the immune stimulant, poly dA:dT, to the DC uptake receptor, DEC205 (DEC). We will show that inoculation of α-DEC-poly dA:dT fusion monoclonal antibodies (mAbs) is sufficient to induce rapid innate cytokine production by DCs. Adaptive T cell immunity is also elicited when the fusion mAb is given in combination with a DC-targeted antigen. Forced entry is not required because α-DEC fusion mAbs deliver the poly dA:dT to its cytosolic targets.
","abstract_html":"<p>Dendritic cells (DCs) have been shown to be efficient and specialized in antigen presentation, but innate stimuli have not been selectively delivered to determine if these cells alone can integrate innate and adaptive immunity in vivo. Here we illustrate a novel method using expressed protein ligation (EPL) to attach the immune stimulant, poly dA:dT, to the DC uptake receptor, DEC205 (DEC). We will show that inoculation of α-DEC-poly dA:dT fusion monoclonal antibodies (mAbs) is sufficient to induce rapid innate cytokine production by DCs. Adaptive T cell immunity is also elicited when the fusion mAb is given in combination with a DC-targeted antigen. Forced entry is not required because α-DEC fusion mAbs deliver the poly dA:dT to its cytosolic targets.</p>","abstract_has_math":false,"creators":["Barbuto, Scott A"],"institution":null,"degree_name":"Doctor of Philosophy (PhD)","degree_level":"Thesis","degree_discipline":null,"degree_department":null,"school":null,"contributors":["Ralph Steinman"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2012,"date_issued":"2012-01-01T08:00:00Z","date_published":"2012-01-01T08:00:00Z","updated_at":"2026-07-24T04:11:05Z","subjects":["dendritic cells","antigen presentation","poly dA:dT","DEC205","innate immunity","adaptive immunity","Life Sciences"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://digitalcommons.rockefeller.edu/student_theses_and_dissertations/243","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Ralph Steinman"]},{"key":"dc:creator","label":"Author","values":["Barbuto, Scott A"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Doctor of Philosophy (PhD)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["dendritic cells","antigen presentation","poly dA:dT","DEC205","innate immunity","adaptive immunity","Life Sciences"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://digitalcommons.rockefeller.edu/student_theses_and_dissertations/243"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Dendritic cells (DCs) have been shown to be efficient and specialized in antigen presentation, but innate stimuli have not been selectively delivered to determine if these cells alone can integrate innate and adaptive immunity in vivo. Here we illustrate a novel method using expressed protein ligation (EPL) to attach the immune stimulant, poly dA:dT, to the DC uptake receptor, DEC205 (DEC). We will show that inoculation of α-DEC-poly dA:dT fusion monoclonal antibodies (mAbs) is sufficient to induce rapid innate cytokine production by DCs. Adaptive T cell immunity is also elicited when the fusion mAb is given in combination with a DC-targeted antigen. Forced entry is not required because α-DEC fusion mAbs deliver the poly dA:dT to its cytosolic targets.
"]},{"key":"dc:title","label":"Title","values":["Induction of Innate and Adaptive Immunity In Vivo by Selective Antibody Targeting of Poly dA:dT and Antigen to Dendritic Cells"]}]}],"canonical_facts":{"dc:contributor":["Ralph Steinman"],"dc:creator":["Barbuto, Scott A"],"dc:description.abstract":["Dendritic cells (DCs) have been shown to be efficient and specialized in antigen presentation, but innate stimuli have not been selectively delivered to determine if these cells alone can integrate innate and adaptive immunity in vivo. Here we illustrate a novel method using expressed protein ligation (EPL) to attach the immune stimulant, poly dA:dT, to the DC uptake receptor, DEC205 (DEC). We will show that inoculation of α-DEC-poly dA:dT fusion monoclonal antibodies (mAbs) is sufficient to induce rapid innate cytokine production by DCs. Adaptive T cell immunity is also elicited when the fusion mAb is given in combination with a DC-targeted antigen. Forced entry is not required because α-DEC fusion mAbs deliver the poly dA:dT to its cytosolic targets.
"],"dc:identifier":["https://digitalcommons.rockefeller.edu/student_theses_and_dissertations/243"],"dc:subject":["dendritic cells","antigen presentation","poly dA:dT","DEC205","innate immunity","adaptive immunity","Life Sciences"],"dc:title":["Induction of Innate and Adaptive Immunity In Vivo by Selective Antibody Targeting of Poly dA:dT and Antigen to Dendritic Cells"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Doctor of Philosophy (PhD)"]},"updated_at":"2026-07-24T04:11:05Z"}