1,25-Dihydroxyvitamin D Regulation of Triacylglycerol Accumulation in Differentiated Adipocytes

Obesity is a major public health concern, both in the United States and worldwide. Therefore, identification of measures by which obesity may be prevented and reversed is of high priority. Epidemiological studies consistently demonstrate an inverse relationship between serum 25(OH)D levels, an indicator of vitamin D status, and measures of adiposity. These data suggest that vitamin D may play a role in the prevention of excessive adiposity. However, whether vitamin D impacts lipid storage and metabolism in terminally differentiated adipocytes is not yet known. The purpose of this work was to determine the impact of 1,25-dihydroxyvitamin D (1,25(OH)2D), the bioactive vitamin D metabolite, on triacylglycerol accumulation and lipid and glucose metabolism in differentiated adipocytes. To study this, 3T3-L1 adipocytes were differentiated for 9 days, followed by stimulation with 1,25(OH)2D (10 nM) or vehicle for 1-7 days. Results indicate that 1,25(OH)2D stimulates a 21% reduction in TAG accumulation in differentiated 3T3-L1 adipocytes after 4 days (P=0.01). This occurs despite a significant increase in fatty acid uptake (P<0.01), assessed using BODIPY FL C16, and with concomitant stimulation of PKA-dependent

Name thesis:degree_name

Doctor of Philosophy (PhD)

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Nutrition Science

Year

2016

Author dc:creator

• Larrick, Brienna

Contributors dc:contributor

• Dorothy Teegarden
• Kimberly K Buhman
• Shawn S Donkin
• Kee-Hong Kim
• Shihuan Kuang

• Adipocyte
• Metabolism
• Obesity
• Triacylglycerol
• Vitamin D

Repository record dc:identifier

https://docs.lib.purdue.edu/open_access_dissertations/1279

OAI identifier oai:identifier

oai:docs.lib.purdue.edu:open_access_dissertations-2495