Macrophage Polarization (M1/M2) and its Role in Wnt5a Secretion/Expression in Atherosclerosis

<p>Macrophages play an important role in the formation of atherosclerotic lesions and their progression. The presence of M1 and M2 macrophages in human atherosclerotic lesions has been suggested. Wnt5a is a noncanonical member of the wingless family of proteins that is expressed in human macrophages in different inflammatory diseases including atherosclerosis. These observations led us to hypothesize that Wnt5a secretion/expression is related to M1 and M2 subsets in atherosclerosis. To investigate this hypothesis, we performed immunohistochemical analysis using Wnt5a, CD68 (for macrophages) and CD163 (for M2 macrophages) in human atherosclerotic tissue sections derived from the carotid arteries of patients undergoing endarterectomy. We also performed immuno-fluorescence analysis to detect Wnt5a in human peripheral blood differentiated M1 and M2 macrophages. </p><p>Our results suggest that Wnt5a secretion/protein expression is mainly associated with the M2 phenotype and shows the participation of other cells in Wnt5a protein expression in the atherosclerotic lesions.</p>

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Biomedical Engineering (Engineering and Technology)

Grantor dc:publisher

Ohio University

Year dc:date

2013

Author dc:creator

• Seelamneni, Harsha

Contributors dc:contributor

• Malgor, Ramiro

• Biomedical Engineering
• Wnt5a expression
• M1/M2 macrophages
• Atherosclerosis
• isolation of Peripheral Blood Monocytes

Statement dc:rights

• unrestricted
• This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.

Language dc:language

English

Repository record dc:identifier

http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1357313250

OAI identifier oai:identifier

oai:etd.ohiolink.edu:ohiou1357313250