In Vivo Studies of the Foreign Body Reaction to Biomedical Polymers

The in vivo foreign body reaction on biomaterials regarding development of foreign body giant cells and formation of fibrous capsules was studied. The first study (chapter II) discusses the development of foreign body reaction with specific lymphocytic immunodeficient mice mainly targeting IL-4 (interleukin-4) cytokine. The experiment consisted of in vivo subcutaneous implants of polyether urethane (PEU) and polyethylene terephthalate (PET) in immunodeficient mice strains. Analysis on polymer surfaces using quantitative calculations such as percent fusion, cell density, and nuclei density described the development of foreign body reaction on biomaterial surface. In addition qualitative assessment was implemented to also describe the development of foreign body reaction that provided similar characterization to the quantitative calculations. The second study (chapter III) focuses on the fibrous capsule formation around biomaterials. Electrospun nanofibers poly(e-caprolactone-co-ethylethylene phosphate) coupled with collagen type I siRNA were subcutaneously implanted. A significant decrease in fibrous capsule thickness at weeks 2 and 4 were shown compared to the control nanofibers.

Name thesis:degree_name

Master of Sciences

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Biomedical Engineering

Grantor dc:publisher

Case Western Reserve University School of Graduate Studies

Year dc:date

2013

Author dc:creator

• Yang, Jung Hoon

Contributors dc:contributor

• Anderson , James

• Biomedical Engineering
• Foreign Body Reaction
• Foreign Body Giant Cell Formation
• Immunodeficient Mice
• siRNA
• Fibrous Capsule down regulation

Statement dc:rights

• unrestricted
• This thesis or dissertation is protected by copyright: some rights reserved. It is licensed for use under a Creative Commons license. Specific terms and permissions are available from this document's record in the OhioLINK ETD Center.

Language dc:language

English

Repository record dc:identifier

http://rave.ohiolink.edu/etdc/view?acc_num=case1364982246

OAI identifier oai:identifier

oai:etd.ohiolink.edu:case1364982246