Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 707 for “"siRNA"”.
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Chemical synthesis of glycosylated oligoribonucleotides for siRNA development
In this study, an efficient methodology for the preparation of carbohydrate-RNA conjugates was established, which involved the use of 3,4~diethoxy-3-cyclobutene-l,2- dione (diethyl squarate) as the linking reagent. First, a glycan moiety containing an amino group reacted with diethyl squarate to …
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Designing nanoparticles for highly efficient endothelial siRNA delivery
… these challenges, nanoparticles have delivered siRNA to hepatocytes after intravenous injections as low as 0.001 mg/kg. By contrast, efficient, durable, and robust silencing in other cell types has remained challenging. Herein we describe 7C I, a low molecular weight polymeric nanoparticle that …
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Evaluation of therapeutic effect of IKK epsilon SiRNA on breast cancer cells and development of a peptide-based SiRNA delivery system
The objective of this dissertation is to develop siRNA-based cancer therapeutics. In chapters 1 and 2, we introduced the mechanism, challenge and promise of RNAi-based therapy, as well as various siRNA delivery systems. In Chapter 3, we examined the therapeutic potential of IKKε siRNA on human …
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Novel cell-penetrating peptide-based vectors for siRNA delivery
Harnessing the RNAi pathway with synthetic siRNA as a potent and selective mode of post-transcriptional gene silencing and has therapeutic potential in personalised medicine; however, the large size and negative charge of siRNA creates a hurdle for intracellular delivery that has thus far limited …
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Self-Assembled Gold Nanoplexes for Cancer-Targeted siRNA Delivery
… Moreover, the Au nanoplexes can protect siRNA from RNase degradation and are stable in cell culture medium.</p> <p>siGLuc-ppp, ssRNA<sub>80</sub>, and siF17 were delivered by the assembled Au nanoplexes, and the results were analyzed by GLuc assay, TB assay, and Luciferase assay. In terms …
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Strategies for combinatorial development of siRNA conjugate delivery systems
… A wide variety of small interfering RNA (siRNA) delivery systems has been studied, including lipid nanoparticles (LNPs) and small molecule conjugates. LNPs are among the most potent and diverse nonviral delivery systems, and they have enjoyed continued improvement in efficacy as new …
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Development and characterization of chemical modifications for siRNA strand selection
Small interfering RNA (siRNA) are non-coding, double-stranded RNA that enable efficient gene silencing through RNA interference (RNAi) mechanisms. The primary effector of RNAi is an RNA-guided nuclease, Ago2, which selects one of the two strands as a guide to form the RNA induced silencing complex …
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Suppression of LPS-induced cytokines/chemokines production by CXCL1 siRNA
… measured via ELISA. In addition, several CXCL1 siRNAs were designed, synthesized and tested as inhibitors. The specificity of CXCL1 siRNA inhibition of LPS-induced proteins was analyzed using ELISA and our hybrid method analysis. We analyzed oral bacteria (P. gingivalis, T. denticola, T. …
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Development of siRNA transporter nanocarriers for the regulation of inflammation
… παθολογιών περιλαμβάνει τη χρήση της τεχνολογίας siRNA για την ειδική αποσιώπηση γονιδίων-στόχων. Ωστόσο, η αποτελεσματική μεταφορά των μορίων siRNA στο εσωτερικό των κυττάρων παραμένει μια πρόκληση, απαιτώντας τη χρήση εξειδικευμένων συστημάτων μεταφοράς, όπως οι λιποσωμικοί νανοφορείς, οι οποίοι …
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Advancing and expanding siRNA and saRNA therapeutics applications through chemical modifications
… and activation. Short interfering RNAs (siRNAs) silence genes, while short activating RNAs (saRNAs) activate them. Both types of strands can be used to create new cancer treatments. However, RNA-based therapies face challenges like instability, off-target effects, and low cell membrane …
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Development of Protein Based PCBP2 siRNA Nanocomplex for Liver Fibrosis Therapy
… this dissertation is to develop a protein-based siRNA nanocomplex for the treatment of alcoholic liver fibrosis. Our laboratory recently discovered that silencing the poly (rC) binding protein 2 (PCBP2) gene in hepatic stellate cells (HSCs) leads to the reversal of the accumulated extracellular …
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RNAi Nanotechnology: A Platform for siRNA Screening and Cancer Gene Therapy
… micro RNA (miRNA), short-interfering RNA (siRNA), small nuclear RNA (snRNA) and many others. The noncoding RNAs, such as siRNA, do not express proteins but have been utilized in a wide range of applications, including RNA interference (RNAi) and the regulation of mRNA expression. These …
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MAPK9: Ein neuer Regulator des Triglyzerid-Stoffwechsels, entdeckt durch siRNA Screening
… of lipid composition resulting from siRNA knock-down. Furthermore, the potential of mass spectrometry as a lipid analysis tool in large-scale studies was evaluated. This method was then applied to an essential family of regulatory proteins, the kinases. Lipid composition of 600 …
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Characterization of Bioreducible Poly(B-amino Ester) Nanoparticles for siRNA Delivery
… nanoparticles (NP) that deliver silencing RNA (siRNA) exclusively to tumor cells for gene knockdown. Presently, nonviral delivery vectors, such as poly(B-amino ester) (PBAE) NP, are beneficial delivery systems because they are less immunogenic and easier to chemically modify than vectors …
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Design, Synthesis, and Evaluation of Oleyl-WRH Peptides for siRNA Delivery
… (W), and histidine (H) have been promising for siRNA delivery. Therefore, to enhance siRNA delivery and efficient silencing of the STAT3 gene, herein, we hypothesized to use of fatty acylation with oleyl chain to our newly designed cell-penetrating peptide (WRH) to evaluate their silencing …
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Identification of regulators in autophagosome formation using image-based siRNA screening
… in this field. Here we conducted a genome-wide siRNA screen using a high-throughput imaging system to identify undiscovered regulators in autophagosome formation. In this study, HEK293 cells stably expressing GFP-DFCP1 (GFP-tagged zinc finger FYVE-type containing 1) were used and amino acid …
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Investigation into synthetic protease-sensitive hydrogels as siRNA nanoparticle delivery vehicles.
… establishment and characterisation of the PEI/siRNA nanoparticle technology in our group. siRNA was found to be fully complexed at a PEI nitrogen to siRNA phosphate ratios of 5:1 and higher. A 10:1 ratio and higher were able to protect the siRNA from RNases present in serum for 7 days with up …
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Correlation Between Computed Equilibrium Secondary Structure Free Energy and siRNA Efficiency
… secondary structure of the participating mRNA, siRNA, and their complexes. A previously published data set of 609 experimental points was used for the analysis. While virtually no correlation with the computed structural signatures are observed for individual data points, several clear trends …
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Isoxazole Linked siRNA Cholesterol Conjugates by Catalyst Free Nitrile Oxide/Alkyne Cycloadditions
… the synthesIs of a range of chemically modified siRNAs by catalyst free Click Cycloaddition Chemistry. The first chapter overviews the structure and biological function of RNA, before turning to a discussion on the synthetic value of dipolar cycloaddition chemistry of nitrones and nitrile oxides …
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