INDUCIBLE CELL EXPRESSION OF MMP1A FOR TUNABLE DEGRADATION OF BIOMIMETIC SCAFFOLDS

Therapeutic vascularization remains a challenge in the field of tissue engineering. Designing tissues for long-term success involves finding methods to incorporate functional vascular networks capable of providing nutrients and oxygen and removing wastes from surrounding cells. Recent advances have explored synthetic hydrogel scaffolds aimed at mimicking the ECM to create prevascularized tissues. These hydrogels include cell adhesion peptides, enzyme degradable sequences, and growth factors for endothelial cell homing, matrix remodeling, and proliferation. In order to tune scaffold properties for optimal angiogenic-promoting conditions, material parameters must be adjusted. In this project, a cellular platform was generated to probe a single aspect of the system, enzyme degradable sequences. Cells capable of controllable, inducible expression of Mmp1a were cloned for optimization of collagenase degradable sequence function in PEG-based hydrogels. These cells overexpressed Mmp1a mRNA by QPCR analysis, though further experiments in Western blot and zymography need refining for detecting protein presence and activity.

Name thesis:degree_name

Master of Sciences

Level thesis:degree_level

masters

Discipline thesis:degree_discipline

Biomedical Engineering

Grantor dc:publisher

Case Western Reserve University School of Graduate Studies

Year dc:date

2013

Author dc:creator

• Tsung, Irene

Contributors dc:contributor

• von Recum, Horst

• Engineering
• Biomedical Engineering

Statement dc:rights

• unrestricted
• This thesis or dissertation is protected by copyright: some rights reserved. It is licensed for use under a Creative Commons license. Specific terms and permissions are available from this document's record in the OhioLINK ETD Center.

Language dc:language

English

Repository record dc:identifier

http://rave.ohiolink.edu/etdc/view?acc_num=case1363689452

OAI identifier oai:identifier

oai:etd.ohiolink.edu:case1363689452