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Showing 1 to 20 of 33 for “"Zymography"”.
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Matrix metalloproteinases in the equine systemic inflammatory response: implications for equine laminitis
… and evaluated using western blotting and gelatin zymography. The Biotrak MMP-9 Activity Assay was evaluated for use with equine samples using equine neutrophil MMP-9 as a standard, and was determined to have insufficient sensitivity for equine MMP-9. Therefore, zymography was used for evaluating …
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In-vitro assessment of modified resin adhesive-tooth interfaces
… measurement of MMP activity and substrate zymography, respectively. Micro-tensile bond strength and micro-permeability were used to evaluate the modified adhesives’ physical properties. Micro-Raman spectroscopy analysis was validated on carious dentine and it was used to evaluate the …
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The Possible Role for Basigin Receptor Function in The Induction of Matrix Metalloproteinase Expression in Human Endometrial Stromal Cells
… and activation using immunoblotting and zymography. I found the highest expression fold changes in MMP-1, -2, and -9 at 6 hours, and MMP-2 at 24 hours. Zymography showed no difference in time. Future work is recommended to pinpoint peak protein expression for MMPs in HESCs following …
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INDUCIBLE CELL EXPRESSION OF MMP1A FOR TUNABLE DEGRADATION OF BIOMIMETIC SCAFFOLDS
… though further experiments in Western blot and zymography need refining for detecting protein presence and activity.
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Bridging the lesioned spinal cord using hydrogel
… dimensional reconstruction of lesion cavity 7 6. Zymography 7 7. Behavioral assessment 8 III. RESULTS 10 1. Hydrogel injection prevent cavity formation after contusive spinal cord injury 10 2. Hydrogel injection suppress microglial activation 4 week after injection 14 3. Remodelling of …
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Staphylococcus aureus의 Lipoteichoic acid(LTA)에 의한 사람의 정상 코 점막 상피세포에서의 MMP와 TIMP의 발현
… immunosorbent assay(ELISA) F. Gelatin zymography 1. Gelatinase B의 준비, 활성화 2. Gelatinase B의 전기영동, 기질-효소 간 반응 3. 발현된 band의 염색, 결과의 수치화 G. Western blot 1. LTA 처리, 단백질 정량 2. 단백질의 전기영동, Antibody 반응 H. MEK1/2 inhibitor에 의한 MMP-9의 발현 억제 확인 Ⅲ. 결과 A. LTA에 의한 코 점막 상피세포의 세포독성 확인 B. MMP-9와 TIMP-1, …
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TIS21 inhibits cell migration by downregulating expression of matrix metalloproteinases
… dermal fibroblasts (HDF) cells, as confirmed by zymography, RT-PCR. Moreover we demonstrate that TIS21 decreases transcription factor (c-Fos, PEA3) of MMPs. We are going to examine the regulatory mechanism of TIS21-mediated MMPs proteoytic activity.
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Lipoteichoic acid from Staphylococcus aureus induced expression of MMP-9 in human middle ear epithelial cells
… signal transduction were examined by PCR, ELISA, zymography and western blot. LTA isolated from S. aureus increased MMP-9 mRNA expression and secretion in HMEEC, whereas no effect on the expressions of MMP-1, 2, 3, 7 and TIMP-1, 2 was observed. Only the extracellular signal-regulated kinases (ERK) …
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Matrix Metalloproteinases 2 and 9 in Normal Canine Cerebrospinal Fluid
… MMP analysis was done using gelatin zymography and an enzyme linked immunosorbent assay (ELISA). Bands of enzyme activity were visible following staining due to enzyme degradation of the gelatin. A commercially available polyclonal sandwich ELISA was used to identify the pro form of …
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Production of Extracellular Matrix-Degrading Proteases by a Rat B Cell Line.CRL-1631
… MMP-2 and MMP-9 activity was verified by gelatin zymography. TIMP-1, TIMP-2 and uPAR protein expression was confirmed by Western blot analyses. I also report, for the first time, MT -1 MMP gene and protein expression in B cells by RT-PCR and Western blot, respectively. CRL-1631 invasion through …
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Astaxanthin Decreases Invasion and MMP Activity in Triple Negative Breast Cancer Cells
… but does not affect proliferation. Using zymography we showed that 4T1 cells treated with Astaxanthin demonstrate decreased MMP-2, -9, and -13 activities. Western blot analysis of conditioned media and lysates suggests that decreased MMP activity results from decreased protein expression. …
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Therapeutic potential of the anti-fibrotic drug Suberanilohydroxamic acid (SAHA) in canine corneal fibrosis
… signaling proteins. Real-time PCR and zymography quantified matrix metalloproteinases (MMP) mRNA and protein expression, respectively. In study 2, a corneal alkali burn was created in Beagles using 1 N NaOH topically. Dogs were randomly and equally assigned into 2 groups: A) vehicle …
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Met Inactivation by S-allylcysteine Suppresses Migration and Invasion of Nasopharyngeal Cancer Cells Induced by hepatocyte growth factor
… colony forming, western blotting, and gelatin zymography assays were performed in the human nasopharyngeal cancer cell lines HNE1 and HONE1 treated with SAC (0, 10, 20, or 40 mM) and hepatocyte growth factor (HGF). This study showed that SAC could suppress the migration and invasion of HNE1 and …
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Characterization of proteins and tissue remodeling components in porcine aqueous humor
… MMP activity and glaucoma risk factors. Gelatin zymography identified MMPs at 66 kD and approximately 105 kD. The concentration of the 66 kD band compared to human MMP-2 standard was 0.22 ± 0.06 ng/ml for the adult female (AF) samples and 0.28 ± 0.04 ng/ml for the juvenile samples. This …
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Effects of systemic flunixin meglumine, topical oxytetracycline, and topical prednisolone acetate on tear film proteinases innormal horses
… proteinase activity was measured using gelatin zymography and measurements of optical density. Statistical analysis of the difference between the treated and untreated eyes and the eyes before and after treatment was performed using mixed effects model for ANOVA. When eyes were compared after …
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Characterisation of dysregulated proteins in macrophages infected with Mycobacterium smegmatis focusing on matrix metalloproteases and their effectors
… points. The activity of MMPs was analysed by zymography. The activity of MMP-1 and MMP-9 were specifically determined using an MMP-1 fluorogenic assay and a non-fluorogenic MMP-9 substrate monitored using the HPLC. Discovery proteomics was performed for the 18-hour time point with the use of …
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Potential roles for transforming growth factor-B during terminal differentiation of ocular lens cells /
… matrix degrading enzymes (MMPs) was examined by zymography. TGF-β induced MMP activity corresponding to MMP2, MMP9 and an unknown MMP at approximate 77 kD molecular weight. Thus, TGF-β does not function to keep CLAP cells withdrawn from the cell cycle or to promote fiber cell differentiation. …
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The role of platelets and leukocytes in endogenous fibrinolysis.
… collagen. The technique of SDS-PAGE with zymography confirmed the presence of t-PA and PAI-1 antigen in rat plasma. Moreover, this approach was subsequently used to show the presence of PAI-1 in rat platelets, and to obtain semi-quantitative estimates of its concentration.
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Regulation of Matrix Metalloproteinases and Their Inhibitors by DNA Methylation in Human Gestational Tissues
… and -9 activities were evaluated by gelatin zymography and found to be increased in placenta and amnion at specific time points and treatments, correlating with mRNA expression levels. Interrogation of the TIMP-1 and MT1-MMP promoters using Sequenom™ EpiTyper® MassARRAY revealed general …
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Expression of Epidermal Growth Factor Receptor in hypopharyngeal cancer and Effect of EGF in Progression of hypopharyngeal cancer
… by the membrane invasion assay. RT-PCR and zymography were performed to examine the roles of matrix metalloproteinase-2(MMP-2) and MMP-9, as well as the relationship between EGF and MMPs in FaDu invasiveness. Results: The positive rates of EGFR expression in hypopharyngeal SCC were 63.2%. …
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