{"id":{"repo_id":"ohiolink","oai_identifier":"oai:etd.ohiolink.edu:case1363689452"},"canonical_url":"https://search.dev.ndltd.org/etd/ohiolink/oai:etd.ohiolink.edu:case1363689452","repository":{"repo_id":"ohiolink","name":"OhioLINK","base_url":"https://etd.ohiolink.edu/acprod/odb_etd/ws/oai/oai"},"display":{"title":"INDUCIBLE CELL EXPRESSION OF MMP1A FOR TUNABLE DEGRADATION OF BIOMIMETIC SCAFFOLDS","abstract":"Therapeutic vascularization remains a challenge in the field of tissue engineering. Designing tissues for long-term success involves finding methods to incorporate functional vascular networks capable of providing nutrients and oxygen and removing wastes from surrounding cells. Recent advances have explored synthetic hydrogel scaffolds aimed at mimicking the ECM to create prevascularized tissues. These hydrogels include cell adhesion peptides, enzyme degradable sequences, and growth factors for endothelial cell homing, matrix remodeling, and proliferation. In order to tune scaffold properties for optimal angiogenic-promoting conditions, material parameters must be adjusted. In this project, a cellular platform was generated to probe a single aspect of the system, enzyme degradable sequences. Cells capable of controllable, inducible expression of Mmp1a were cloned for optimization of collagenase degradable sequence function in PEG-based hydrogels. These cells overexpressed Mmp1a mRNA by QPCR analysis, though further experiments in Western blot and zymography need refining for detecting protein presence and activity.","abstract_html":"Therapeutic vascularization remains a challenge in the field of tissue engineering. Designing tissues for long-term success involves finding methods to incorporate functional vascular networks capable of providing nutrients and oxygen and removing wastes from surrounding cells. Recent advances have explored synthetic hydrogel scaffolds aimed at mimicking the ECM to create prevascularized tissues. These hydrogels include cell adhesion peptides, enzyme degradable sequences, and growth factors for endothelial cell homing, matrix remodeling, and proliferation. In order to tune scaffold properties for optimal angiogenic-promoting conditions, material parameters must be adjusted. In this project, a cellular platform was generated to probe a single aspect of the system, enzyme degradable sequences. Cells capable of controllable, inducible expression of Mmp1a were cloned for optimization of collagenase degradable sequence function in PEG-based hydrogels. These cells overexpressed Mmp1a mRNA by QPCR analysis, though further experiments in Western blot and zymography need refining for detecting protein presence and activity.","abstract_has_math":false,"creators":["Tsung, Irene"],"institution":"Case Western Reserve University School of Graduate Studies","degree_name":"Master of Sciences","degree_level":"masters","degree_discipline":"Biomedical Engineering","degree_department":null,"school":null,"contributors":["von Recum, Horst"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2013,"date_issued":"2013-03-19","date_published":"2013-03-19","updated_at":"2026-07-24T03:37:01Z","subjects":["Engineering","Biomedical Engineering"],"languages":["English"],"rights":["unrestricted","This thesis or dissertation is protected by copyright: some rights reserved. It is licensed for use under a Creative Commons license. 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