Interactions between adult human mesenchymal stem cells and nanofibrous scaffolds of different compositions

Stem cells have become increasingly important in the biomedical field because they have the potential to invade and regenerate damaged tissue. In particular, the bone marrow contains meesenchymal stem cells (MSCs) that have the capacity to differentiate into many cell lineages. The incorporation of MSCs within a multidimensional biomaterial provides a delivery vehicle to areas of injury. Natural and synthetic biomaterials can be made into scaffolds to represent different types of tissues. The ratio of synthetic to natural biomaterials in the scaffold influences the interaction with MSCs. This thesis created two different nanofibrous scaffolds by a process of electrospinning. The scaffolds consisted of a nanofiber mesh mimicking the extracellular matrix. One is derived solely from Poly-L-lactide acid and the other from Poly-L-lactide/Gelatin (50:50). Before and after culturing, measurements of fiber diameter, thermodynamics, and tensile strength were collected. Studies with primary human MSCs were conducted to characterize the in vitro behavior of MSCs seeded on and adhered to scaffolds of different compositions.

Name thesis:degree_name

Master of Science in Biomedical Engineering - (M.S.)

Discipline thesis:degree_discipline

Biomedical Engineering

Year

2008

Author dc:creator

• Moore, Lisamarie

Contributors dc:contributor

• Pranela Rameshwar
• Michael Jaffe
• Treena Livingston Arinzeh

• Mesenchymal stem cells
• nanofibrous scaffolds
• Biomedical Engineering and Bioengineering

Repository record dc:identifier

https://digitalcommons.njit.edu/theses/360

OAI identifier oai:identifier

oai:digitalcommons.njit.edu:theses-1359