Novel Physical Interactions of Vps1 with Major Intracellular Traffic Regulators

Vacuolar Protein Sorting 1 (Vps1), the yeast homolog to human dynamin, is a GTPase that acts as a pivotal component in membrane trafficking. In light of observations that VPS1 displayed positive genetic interactions with four genes implicated in the recycling traffic (YPT6, GYP1, RCY1, and RIC1) and with seven ESCRT (Endosomal Sorting Complex Required for Transport) components in the degradation traffic (VPS22, 23, 24, 25, 28, 36, and 37), I investigated the possibility of the physical interactions of Vps1 with the eleven regulators, using a yeast two-hybrid system, and identified novel Vps1 binding partners, including Ypt6, Vps22, Vps24, and Vps36. The GTPase and middle domain of Vps1 appeared to have higher affinities to Ypt6 than the full-length Vps1, whereas the full length of Vps1 had a higher binding affinity to Vps22 than shorter fragments of Vps1. I also provide evidence from an overexpression assay that Vps1 functions downstream of Ypt6. According to the qRT-PCR analysis, the mRNA expression levels of traffic factors were not affected in the absence of VPS1. In addition, the yeast two-hybrid genome-wide screening results revealed Ste24 as a novel binding partner of Vps1. Taken together, my results support an important role of Vps1 in major intracellular trafficking processes, providing novel insight into the activity of Vps1.

Name thesis:degree_name

Master of Science in Biology

Level thesis:degree_level

Masters

Discipline thesis:degree_discipline

Biology

Year

2014

Author dc:creator

• McDermott, Hyoeun Ahn

Contributors dc:contributor

• Kyoungtae Kim

• Vps1
• Ypt6
• ESCRT
• recycling traffic
• degradation traffic
• Biology

Statement dc:rights

• © Hyoeun Ahn McDermott

Repository record dc:identifier

https://bearworks.missouristate.edu/theses/1332

OAI identifier oai:identifier

oai:bearworks.missouristate.edu:theses-2333