Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"ESCRT"”.
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Regulation of The Escrt Function of Alix
<p>The ESCRT (endosomal sorting complex required for transport) is an evolutionary conserved membrane remodeling machinery that performs membrane invagination and abscission. ALIX is a widely expressed adaptor protein that is critically involved in three classical ESCRT-mediated processes, …
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Role And Regulation Of ESCRT-III In Multivesiculr Body Biogenesis
… sorting complex required for transports: ESCRT) machinery responsible for multivesicular body: MVB) biogenesis is essential for receptor downregulation, viral budding and cytokinesis. ESCRT-III is a large polymer built from related ESCRT-III proteins that is thought to help generate …
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Glycoprotein M and ESCRT in herpes simplex virus type 1 assembly
… Sorting Complexes Required for Transport (ESCRT) in secondary envelopment. Firstly, it has been reported that gH/L can be efficiently internalised and targeted to the TGN by the co-expression of gM in transfection assays. In order to examine the role of gM in guiding the localisation of …
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Endosomal Sorting Complex Required for Transport (ESCRT): Investigation of the expression and functional roles in neurodegenerative diseases
Endosomal Sorting Complex Required for Transport (ESCRT) is responsible for trafficking of misfolded proteins, unwanted organelles and ubiquitinated proteins to be degraded by lysosome. Abnormalities in ESCRT proteins have been linked to various neurodegenerative diseases such as Alzheimer's …
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Structural and Functional Characterization of the Endosome-associated Deubiquitinating Enzyme AMSH
… sorting complexes required for transport (ESCRT) machinery is a ubiquitin-dependent molecular mechanism made of up of four individual complexes: ESCRT-0, -I, -II, III, that is necessary for regulating the degradation of cell surface receptors directed towards the lysosome. Not only are the …
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Characterizing Roles of Host Cell Trafficking Factors in HIV Assembly and Release
… of an infected cell, HIV-1 hijacks the cellular ESCRT membrane scission machinery by binding ESCRT-I. In order to probe the organization of ESCRT-I in this process, we used CRISPR/Cas9-mediated genome editing in human cell lines to tag endogenously expressed ESCRT-I with GFP. These knock-in cell …
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Ultrastructural analysis of HIV-1 infection in human cells
… Teil habe ich die Verteilung eines zellulären, ESCRT benannten, Proteinkomplexes analysiert. ESCRT ist essentiell für die Freisetzung von HIV aus infizierten Zellen und spielt sowohl beim Sortieren von Proteinen in die internen Vesikel eines multivesikulären endosomalen Kompartiments …
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CELLULAR AND MOLECULAR PATHWAYS CONTROLLING SARS-COV2 ASSEMBLY AND EGRESS
… on the Endosomal Complex Required for Transport (ESCRT) machinery that appears to behave as an anti-viral factor. To address the mechanism by which this molecular machinery acts as a restriction factor, we first used a reductionist approach based on transfection of single viral structural …
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Structural and biochemical characterisation of pUL7 and pUL51, conserved tegument proteins required for efficient herpesvirus assembly
… sorting complex required for transport (ESCRT)-III components, a class of cellular proteins involved in membrane scission. HSV-1 pUL51 binds lipids when recombinantly expressed, and forms filamentous and ring-shaped polymers that resemble those formed by ESCRT-III superfamily proteins …
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Molecular Mechanisms Regulating Chemokine Receptor CXCR4 Signaling and Trafficking
… sorting complex required for transport (ESCRT) pathway. This pathway consists of four different protein complexes (ESCRT-0, I, II and III), plus several accessory factors, that act in a sequential and coordinated manner to target proteins for lysosomal degradation. Although CXCR4 is …
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Visualizing Protease Activation, Retrochmp3 Activity, and VPR Recruitment During HIV-1 Assembly
… becomes active prior to recruitment of the host ESCRT proteins and scission from the cell. Chapter 3 of this thesis describes my work on the protein retroCHMP3, which was performed in collaboration with Sundquist lab at the University of Utah. The retroCHMP3 protein was originally found in …
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Characterization of The Ubiquitin Ligase, Ube4B, In Endocytic Trafficking
… sorting complexes required for transport (ESCRT) machinery. A subset of ESCRT proteins bind directly to ubiquitin, allowing the sorting machinery to engage and manipulate the movement of protein cargo into inwardly budded MVB vesicles. Lack of a ubiquitin tag precludes EGFR from inclusion …
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Regulation of autophagosome formation and maturation by neurodegeneration-associated proteins
… sorting complex required for transport (ESCRT) complex and then released by dynamin 2 (DNM2) before fusing with lysosomes to enable the degradation of the autophagy substrates. Impaired autophagic clearance is a shared hallmark of neurodegenerative diseases and exacerbates …
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Using Chemical Probes to Examine Cellular Activities
… sorting complex required for transport (ESCRT) to the reforming nuclear envelope, where spastin is proposed to sever stable microtubules. However, we currently lack an understanding of spastin's dynamics during anaphase, particularly the roles of microtubules and ESCRT proteins in said …
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Endocytic Trafficking of The Amyloid Precursor Protein In Rat Cortical Neurons
… Sorting Complexes Required for Transport (ESCRT) machinery. This implied that APP is ubiquitinated to allow ESCRT interactions. We then identified that the endosome-associated E3 ubiquitin ligase, UBE4B, is required for efficient endosomal APP trafficking. These results suggest that the …
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Nouveau rôle pour la ferriréductase Frp1 dans l’acquisition de l’hème par la voie Shu1-dépendante chez la levure Schizosaccharomyces pombe
… à la surface cellulaire via le système ESCRT. Cette propriété suggère l’existence d’une ou de plusieurs protéines transmembranaires partenaires qui participeraient à l’internalisation de Shu1 après sa liaison à l’hème. Parmi les protéines candidates, la ferriréductase Frp1 pourrait jouer …
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The mechanism of cyclic proteasome-mediated protein degradation in Sulfolobus acidocaldarius
… degradation of a non-contractile homologue of ESCRT-III proteins, CdvB (Cell division protein B), while the ESCRT-III homologues CdvB1 and CdvB2 remained stable until G1, enabling them to drive cytokinesis and abscission to completion. Thus, tight control over the timing and specificity of …
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CBL and CBL-B Dictate CSF-1R Endocytic Traffic and Signaling in Macrophages
… on endosome and macropinosome. One of the key ESCRT proteins, HRS, did not associate with the CSF-1R in DKO macrophages indicating Cbl and Cbl-b are required for ESCRT-mediated transport into the lumen of macropinosome. Surprisingly, the CSF-1R was still degraded in DKO cells by an unknown …
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Investigating the Role of Stem-Loop 1 in the Assembly Process of HIV-1
… endosomal complexes required for transport (ESCRT) pathway to enable budding. An ESCRT-associated protein, ALIX, is known to be capable of binding to the nucleocapsid (NC) domain of Gag using lipids or RNA as a ‘bridge’ in vitro. It was hypothesised that SL1 mutation disrupts an RNA-dependent …
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Mechanism and Functions of STING-Induced Noncanonical Autophagy
… noncanonical autophagy induces ALIX-meditated ESCRT machinery recruitment to mitigate endolysosomal perturbation. Our study unraveled the multifaceted functions of STING-induced noncanonical autophagy in regulating endolysosomal homeostasis.
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