Immuno-histochemical method for demonstration of Lactic Dehydrogenase "M" subunits in Rat Skeletal Muscle, Kidney and Peripheral Nerve

<p>Immunolocalization of lactic dehydrogenase (LDH) rrM'‘' sub units has been achieved using paraformaldehyde vapor phase fixation of freeze-dried sections. An antiserum to monkey LDH-M^ was found to cross react with rat LDH-M subunits. The fibers of the gastrocnemius and soleus muscles which are stained using the immuno-peroxidase procedure do not stain in the presence of 4 M urea in the conventional method. In kidney, the collecting tubules are the structures which stain most intensely with the immuno-peroxidase procedure and in peripheral nerve both the Schwann cells and axons stain. The nuclei of muscle fibers. kidney tubular cells and Schwann cells are clearly stained using the immuno-peroxidase procedure, whereas they do not stain with the conventional tetrazolium method for LDH.</p>

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Anatomy

Year

1976

Author dc:creator

• Bustos-Arango, Luis M.

Contributors dc:contributor

• Paul J. McMillan
• William M. Hooker
• Donald D. Rafuse

• Anatomy
• Lactate Dehydrogenase

Statement dc:rights

• This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights.

Language dc:language

English

Repository record dc:identifier

https://scholarsrepository.llu.edu/etd/645

OAI identifier oai:identifier

oai:scholarsrepository.llu.edu:etd-1691