Immunolocalization of lactic dehydrogenase (LDH) rrM'‘' sub units has been achieved using paraformaldehyde vapor phase fixation of freeze-dried sections. An antiserum to monkey LDH-M^ was found to cross react with rat LDH-M subunits. The fibers of the gastrocnemius and soleus muscles which are stained using the immuno-peroxidase procedure do not stain in the presence of 4 M urea in the conventional method. In kidney, the collecting tubules are the structures which stain most intensely with the immuno-peroxidase procedure and in peripheral nerve both the Schwann cells and axons stain. The nuclei of muscle fibers. kidney tubular cells and Schwann cells are clearly stained using the immuno-peroxidase procedure, whereas they do not stain with the conventional tetrazolium method for LDH.
","abstract_html":"<p>Immunolocalization of lactic dehydrogenase (LDH) rrM'‘' sub units has been achieved using paraformaldehyde vapor phase fixation of freeze-dried sections. An antiserum to monkey LDH-M^ was found to cross react with rat LDH-M subunits. The fibers of the gastrocnemius and soleus muscles which are stained using the immuno-peroxidase procedure do not stain in the presence of 4 M urea in the conventional method. In kidney, the collecting tubules are the structures which stain most intensely with the immuno-peroxidase procedure and in peripheral nerve both the Schwann cells and axons stain. The nuclei of muscle fibers. kidney tubular cells and Schwann cells are clearly stained using the immuno-peroxidase procedure, whereas they do not stain with the conventional tetrazolium method for LDH.</p>","abstract_has_math":false,"creators":["Bustos-Arango, Luis M."],"institution":null,"degree_name":"Master of Science (MS)","degree_level":"Thesis","degree_discipline":"Anatomy","degree_department":null,"school":null,"contributors":["Paul J. McMillan","William M. Hooker","Donald D. Rafuse"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":1976,"date_issued":"1976-06-01T07:00:00Z","date_published":"1976-06-01T07:00:00Z","updated_at":"2026-07-24T02:52:59Z","subjects":["Anatomy","Lactate Dehydrogenase"],"languages":["English"],"rights":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://scholarsrepository.llu.edu/etd/645","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Paul J. McMillan","William M. Hooker","Donald D. Rafuse"]},{"key":"dc:creator","label":"Author","values":["Bustos-Arango, Luis M."]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Anatomy"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MS)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Anatomy","Lactate Dehydrogenase"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]},{"key":"dc:rights","label":"Dc Rights","values":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://scholarsrepository.llu.edu/etd/645"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Immunolocalization of lactic dehydrogenase (LDH) rrM'‘' sub units has been achieved using paraformaldehyde vapor phase fixation of freeze-dried sections. An antiserum to monkey LDH-M^ was found to cross react with rat LDH-M subunits. The fibers of the gastrocnemius and soleus muscles which are stained using the immuno-peroxidase procedure do not stain in the presence of 4 M urea in the conventional method. In kidney, the collecting tubules are the structures which stain most intensely with the immuno-peroxidase procedure and in peripheral nerve both the Schwann cells and axons stain. The nuclei of muscle fibers. kidney tubular cells and Schwann cells are clearly stained using the immuno-peroxidase procedure, whereas they do not stain with the conventional tetrazolium method for LDH.
"]},{"key":"dc:title","label":"Title","values":["Immuno-histochemical method for demonstration of Lactic Dehydrogenase \"M\" subunits in Rat Skeletal Muscle, Kidney and Peripheral Nerve"]}]}],"canonical_facts":{"dc:contributor":["Paul J. McMillan","William M. Hooker","Donald D. Rafuse"],"dc:creator":["Bustos-Arango, Luis M."],"dc:description.abstract":["Immunolocalization of lactic dehydrogenase (LDH) rrM'‘' sub units has been achieved using paraformaldehyde vapor phase fixation of freeze-dried sections. An antiserum to monkey LDH-M^ was found to cross react with rat LDH-M subunits. The fibers of the gastrocnemius and soleus muscles which are stained using the immuno-peroxidase procedure do not stain in the presence of 4 M urea in the conventional method. In kidney, the collecting tubules are the structures which stain most intensely with the immuno-peroxidase procedure and in peripheral nerve both the Schwann cells and axons stain. The nuclei of muscle fibers. kidney tubular cells and Schwann cells are clearly stained using the immuno-peroxidase procedure, whereas they do not stain with the conventional tetrazolium method for LDH.
"],"dc:identifier":["https://scholarsrepository.llu.edu/etd/645"],"dc:language":["English"],"dc:rights":["This title appears here courtesy of the author, who has granted Loma Linda University a limited, non-exclusive right to make this publication available to the public. The author retains all other copyrights."],"dc:subject":["Anatomy","Lactate Dehydrogenase"],"dc:title":["Immuno-histochemical method for demonstration of Lactic Dehydrogenase \"M\" subunits in Rat Skeletal Muscle, Kidney and Peripheral Nerve"],"thesis:degree_discipline":["Anatomy"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science (MS)"]},"updated_at":"2026-07-24T02:52:59Z"}