Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 36 for “"immunolocalization"”.
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Purification, application and immunolocalization of thermostable xylanases
Submitted in fulfillment of the requirements of the degree of Master of Technology (Biotechnology), Durban University of Technology, Durban, South Africa, 2014.
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Immunolocalization of dystrophin and neurofilament protein in muscle spindles of normal, mdx-dystrophic, and denervated mice
Dystrophin is a high molecular weight protein localized under the sarcolemma of normal extrafusal muscle fibers but absent in skeletal muscles of Duchenne muscular dystrophy patients and in the mdx mouse. Muscle spindles in the soleus of 32-week-old normal and age-matched mdx mice were examined by …
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Molecular cloning, immunolocalization, and transgenic expression in yeast and tobacco of three cDNAs that encode sugar transporters in sugar beet
Sugar transporter genes from sugar beet have been cloned as a first step in understanding their contribution to assimilate partitioning. Two cloning strategies were attempted. One was functional complementation of a yeast mutant with a sugar beet cDNA library constructed in a yeast expression …
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Characterization of the expression patterns of <i>CELLULOSE SYNTHASE-LIKE A (CSLA)</i> genes in <i>arabidopsis thaliana</i> using reporter promoter-fusions and immunolocalization
<p>Plant cell walls are the most abundant source of renewable biomass on Earth. Cell wall carbohydrates have many practical applications (e.g., forage, building materials, biofuels, textiles, paper, etc.), and within plants they contribute to structure, provide defense, and facilitate cellular …
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Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis
… in determination of expression conditions and immunolocalization studies.
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Immuno-histochemical method for demonstration of Lactic Dehydrogenase "M" subunits in Rat Skeletal Muscle, Kidney and Peripheral Nerve
<p>Immunolocalization of lactic dehydrogenase (LDH) rrM'‘' sub units has been achieved using paraformaldehyde vapor phase fixation of freeze-dried sections. An antiserum to monkey LDH-M^ was found to cross react with rat LDH-M subunits. The fibers of the gastrocnemius and soleus muscles which are …
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Junction Specificity and Transmembrane Linkages of Integrins
… in the synaptic vesicle fusion core complex. Immunolocalization of P4B2 antigen in developing cultured muscle cells showed that the antigen is incorporated into Z-lines soon after the sarcomeric architecture was positive for $\alpha$-actinin. Together, the data indicate the P4B2 antigen is …
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Characterization of RYamide neuropeptides and their Receptor in the Disease Vector Mosquito, Aedes aegypti
… by structurally-distinct neuropeptides. RYa-1 immunolocalization was found in the central nervous system, midgut, pyloric valve, ileum, rectal papillae, and surprisingly the seminal vesicles. RYamide and its receptor transcript analysis revealed potential regulatory effects of the digestive, …
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Isolation and Characterization of D-Myo-Inositol-3-Phosphate Synthase Gene Family Members in Soybean
… abundantly expressed early in seed development. Immunolocalization of MIPS protein in developing seeds confirmed expression of MIPS early in seed development and correlated MIPS protein accumulation in soybean seed tissue with tissues in which phytic acid is known to accumulate. The promoter …
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Identification and characterization of GRP50 : a novel Golgi-associated ankyrin repeat protein
… protein-protein interaction module. We have used immunolocalization techniques to show that GRP50 is peripherally associated with the cytoplasmic face of Golgi and vesicle membranes in cultured mammalian fibroblasts. Further, we have demonstrated that Golgi-association of GRP50 is sensitive to the …
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Hemodynamic regulation of blood-brain barrier phenotype: A cooperative effort linking adherens and tight junction complexes
… junction protein expression and enhance protein immunolocalization at the cell-cell border, in parallel with reduced paracellular solute flux. For these studies, cultured bovine brain microvascular endothelial cells (BBMvEC’s) were exposed to laminar shear stress (0-10 dynes/cm 2, 0-24 h) in …
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Investigation of the roX RNAs and the RNA Helicase MLE in Dosage Compensation in Drosophila melanogaster
… the effects of mutants and mutant transgenes, immunolocalization experiments to study MSL protein and roX RNA to chromosomes. For the first time in vivo, we demonstrate that MLE associates with double stranded RNA in a sequence non-specific manner that is independent of other DCC components. …
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The role of SYN1 in early Arabidopsis meiosis
… meiocytes. To investigate this further, immunolocalization studies in wild-type and syn1\(^{-/-}\) were conducted. Distribution of ASY1 and AtZYP1 was affected in syn1\(^{-/-}\). Both proteins appeared as aggregates, developing into an abnormal short linear signal in early prophase I, …
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Development of antibodies for characterizing the Arabidopsis flavonoid biosynthetic pathway
… experiments addressing Western blot analysis, immunolocalization experiments, and disruption of the flavonoid biosynthetic pathway by introduction of the corresponding genes into transgenic Arabidopsis plants.
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THE ROLE OF SKELETAL MUSCLE-SYNTHESIZED BRAIN DERIVED NEUROTROPHIC FACTOR IN THE MAINTENANCE OF MOTOR NEURON MITOCHONDRIAL POPULATIONS
… microscopy and IMARIS 3D rendering software. Immunolocalization was measured throughout the axon and axon terminals of motorneurons. We found no significant difference in mitochondrial populations within the pre-or post-synapse of control and msBDNF deficient animals. Results indicate that …
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Role of the Nfa1 Protein in Pathogenic Naegleria fowleri Co-cultured with Target Cells
… antibody, revealed pseudopodia-specific immunolocalization of Nfa1 protein in a trophozoite of N. fowleri. An anti-Nfa1 antibody showed a neutralizing effect on the cytotoxicity of N. fowleri trophozoites against CHO cells, much like treating an anti-Nfa1 antibody on a cocultivating …
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Molecular genetic characterization of the Drosophila synaptotagmin family
… mammalian Synaptotagmins 4, 7, 12 and 14. Using immunolocalization and in situ hybridization experiments (Chapter 2), we demonstrate that each isoform has a unique subcellular localization and expression pattern, suggesting only Synaptotagmin 1 functions in synaptic vesicle exocytosis. Consistent …
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Molecular Genetics and Subcellular Localization of Flavonoid Metabolism in Arabidopsis
… biosynthesis, CHS and chalcone isomerase (CHI), immunolocalization experiments were performed. Results indicate that CHS and CHI are abundant in epidermal and cortex cells of the root elongation zone and the root tip, consistent with the accumulation of flavonoid endproducts at these sites. At …
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