Lethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry
Elucidating the role of eukaryotic initiation factor 5B (eIF5B) in non-canonical translation initiation
Abstract
dc:description.abstractCap-dependent translation drives the global synthesis of proteins. Under stress conditions global protein production is attenuated, yet the translation of ATF4 is upregulated through uORF- mediated translation initiation. eIF5B has been shown to deliver initiator-tRNA during non-canonical translation initiation. As such, we defined the role of eIF5B in the non-canonical translation of ATF4, and p27. Through polysome profiling and luciferase reporter assays, we confirmed that eIF5B facilitates uORF2-mediated repression of ATF4 translation. We determined that eIF5B has transcriptome-wide effects on signaling pathways, verifying activation of the JNK arm of the MAPK pathway and upregulation of dyskerin. I investigated the role eIF5B has in regulation of p27, and suggest that the mechanism is IRES-dependent. This study furthers the understanding into mechanisms of alternative translation initiation, which is critical to gene expression regulation.
Degree
thesis:*- Grantor dc:publisher
- Lethbridge, Alta. : University of Lethbridge, Dept. of Chemistry and Biochemistry
- Year dc:date.issued
- 2019
Author and committee
dc:creator, dc:contributor.*- Authors dc:creator
-
- Bressler, Kamiko R.
- University of Lethbridge. Faculty of Arts and Science
- Advisor dc:contributor.supervisor
-
- Thakor, Nehal
Subjects
dc:subject × 3Rights
- Language dc:language.iso
- en_CA
Identifiers
dc:identifier.*- Identifier
- hdl:10133/5666