Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"uORF"”.
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Translational Regulation in the 5′UTR of Enteroviruses
… polyprotein ORF (ppORF) and the upstream ORF (uORF). The uORF encodes a small protein, UP. In addition to the canonical uORF start codon (uAUG), situated on the domain six (dVI) stem loop structure within the viral internal ribosome entry site (IRES), some viruses have additional AUGs both up …
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Comparative analysis of African and Asian/American Zika virus strains at the transcriptional and translational level
… overlooked upstream open reading frames (uORFs) in the 5′ untranslated region (UTR) of the American ZIKV genome were identified, initiating at non-canonical CUG and UUG codons; and a single uORF in the 5′UTR of the African ZIKV, initiating at a CUG start codon. The uORFs in the American …
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The control of embryogenic competence in Alfalfa (Medicago falcata L.)
… this gene was the presence of a highly conserved uORF sequence. In order to characterise the role of this gene in somatic embryogenesis, constructs carrying different fragments of the gene in sense and antisense orientations were established and introduced into alfalfa plants via …
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A multi-level approach to understanding the regulation of translation initiation
… insufficiency, and present evidence of a novel uORF in murine ATF4 whose ribosome occupancy is regulated by the presence of amino acids. We identify the 4EBPs as the mTORC1 substrates that mediate the major effects of mTORC1 inhibition on translation of mRNAs both globally and on 5'TOP mRNAs …
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The response of eIF2α kinases to plant virus infection
… that contain an upstream open reading frame (uORF) are preferentially translated. In plants, two types of eIF2α kinases have been reported - general control non-derepressible-2 kinase (GCN2) and protein kinase RNA-activated (PKR). The aim of this research was to further elucidate the role of …
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Ribosome profiling of porcine reproductive and respiratory syndrome virus reveals novel features of viral gene expression
… a highly translated, short upstream ORF (uORF) was identified in the 5′ UTR, the presence of which is highly conserved amongst PRRSV-2 isolates. This work is the first application of RiboSeq to arterivirus-infected cells and reveals new features which add to our understanding of the …
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The mechanisms regulating the transcription factor ATF5 and its function in the integrated stress response
… involves two upstream open reading frames (uORFs) located in the 5′-leader of the ATF5 mRNA, a feature shared with ATF4. Mutational analyses of the 5′-leader of ATF5 mRNA fused to a luciferase reporter suggests that the 5′-proximal uORF1 is positive-acting, allowing scanning ribosomes to …
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The Regulation of NAP4 in Saccharomyces cerevisiae
… via two short upstream open reading frames (uORFs) in the 5' leader sequence of HAP4 mRNA; (2) proteasome-dependent degradation of Hap4; and (3) identification of two negative regulators of HAP4 expression, CYC8 and SIN4. I also report differential patterns of Hap4 cellular localization which …