Interaction of akirin and pannier during embryogenesis of Drosophila melanogaster

<p><em>Akirin</em>, a highly conserved protein found in nearly all eukaryote species, is a transcriptional cofactor that has been known to play a critical role in the muscular development in <em>Drosophila</em>. In this study, we identified and analyzed the genetic interaction of <em>akirin </em>with the transcriptional GATA factor, <em>pannier</em>. Also, we verified whether two (a third region consisting of a combination of both intronic regions) highly conserved intronic regions of <em>akirin</em> could be likely <em>akirin </em>regulatory elements by studying their expression pattern in <em>Drosophila melanogaster</em>. Acknowledging <em>pannier’s</em> role in the early stages of somatic muscular development, a genetic cross of <em>akirin/pannier</em> double heterozygous mutant embryos were imaged to visualize somatic muscular phenotype. Using a reporter gene in <em>Drosophila</em> embryos, we tested whether these identified regulatory elements are indeed <em>akirin </em>regulators. The results indicated that the genetic interaction of <em>akirin</em> and <em>pannier</em> is crucial for the proper development of somatic muscles and help identify the expression pattern of the potential <em>akirin</em> regulators in embryos.</p>

Name thesis:degree_name

Master of Science in Integrative Biology (MSIB)

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Biology

Year dc:date.available

2022

Author dc:creator

• Wandji, Georgette-vanelle

Contributors dc:contributor

• Dr. Scott Nowak
• Dr. Anton Bryantsev
• Dr. Carol Chrestensen

• akirin
• Biology
• Integrative Biology

Repository record dc:identifier

https://digitalcommons.kennesaw.edu/integrbiol_etd/88

OAI identifier oai:identifier

oai:digitalcommons.kennesaw.edu:integrbiol_etd-1090