Akirin, a highly conserved protein found in nearly all eukaryote species, is a transcriptional cofactor that has been known to play a critical role in the muscular development in Drosophila. In this study, we identified and analyzed the genetic interaction of akirin with the transcriptional GATA factor, pannier. Also, we verified whether two (a third region consisting of a combination of both intronic regions) highly conserved intronic regions of akirin could be likely akirin regulatory elements by studying their expression pattern in Drosophila melanogaster. Acknowledging pannier’s role in the early stages of somatic muscular development, a genetic cross of akirin/pannier double heterozygous mutant embryos were imaged to visualize somatic muscular phenotype. Using a reporter gene in Drosophila embryos, we tested whether these identified regulatory elements are indeed akirin regulators. The results indicated that the genetic interaction of akirin and pannier is crucial for the proper development of somatic muscles and help identify the expression pattern of the potential akirin regulators in embryos.
","abstract_html":"<p><em>Akirin</em>, a highly conserved protein found in nearly all eukaryote species, is a transcriptional cofactor that has been known to play a critical role in the muscular development in <em>Drosophila</em>. In this study, we identified and analyzed the genetic interaction of <em>akirin </em>with the transcriptional GATA factor, <em>pannier</em>. Also, we verified whether two (a third region consisting of a combination of both intronic regions) highly conserved intronic regions of <em>akirin</em> could be likely <em>akirin </em>regulatory elements by studying their expression pattern in <em>Drosophila melanogaster</em>. Acknowledging <em>pannier’s</em> role in the early stages of somatic muscular development, a genetic cross of <em>akirin/pannier</em> double heterozygous mutant embryos were imaged to visualize somatic muscular phenotype. Using a reporter gene in <em>Drosophila</em> embryos, we tested whether these identified regulatory elements are indeed <em>akirin </em>regulators. The results indicated that the genetic interaction of <em>akirin</em> and <em>pannier</em> is crucial for the proper development of somatic muscles and help identify the expression pattern of the potential <em>akirin</em> regulators in embryos.</p>","abstract_has_math":false,"creators":["Wandji, Georgette-vanelle"],"institution":null,"degree_name":"Master of Science in Integrative Biology (MSIB)","degree_level":"Thesis","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Dr. Scott Nowak","Dr. Anton Bryantsev","Dr. Carol Chrestensen"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2022,"date_issued":"2022-08-10T07:00:00Z","date_published":"2022-08-10T07:00:00Z","updated_at":"2026-07-24T02:43:58Z","subjects":["akirin","Biology","Integrative Biology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://digitalcommons.kennesaw.edu/integrbiol_etd/88","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Dr. Scott Nowak","Dr. Anton Bryantsev","Dr. Carol Chrestensen"]},{"key":"dc:creator","label":"Author","values":["Wandji, Georgette-vanelle"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2027-08-09T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science in Integrative Biology (MSIB)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["akirin","Biology","Integrative Biology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://digitalcommons.kennesaw.edu/integrbiol_etd/88"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Akirin, a highly conserved protein found in nearly all eukaryote species, is a transcriptional cofactor that has been known to play a critical role in the muscular development in Drosophila. In this study, we identified and analyzed the genetic interaction of akirin with the transcriptional GATA factor, pannier. Also, we verified whether two (a third region consisting of a combination of both intronic regions) highly conserved intronic regions of akirin could be likely akirin regulatory elements by studying their expression pattern in Drosophila melanogaster. Acknowledging pannier’s role in the early stages of somatic muscular development, a genetic cross of akirin/pannier double heterozygous mutant embryos were imaged to visualize somatic muscular phenotype. Using a reporter gene in Drosophila embryos, we tested whether these identified regulatory elements are indeed akirin regulators. The results indicated that the genetic interaction of akirin and pannier is crucial for the proper development of somatic muscles and help identify the expression pattern of the potential akirin regulators in embryos.
"]},{"key":"dc:title","label":"Title","values":["Interaction of akirin and pannier during embryogenesis of Drosophila melanogaster"]}]}],"canonical_facts":{"dc:contributor":["Dr. Scott Nowak","Dr. Anton Bryantsev","Dr. Carol Chrestensen"],"dc:creator":["Wandji, Georgette-vanelle"],"dc:date.available":["2027-08-09T07:00:00Z"],"dc:description.abstract":["Akirin, a highly conserved protein found in nearly all eukaryote species, is a transcriptional cofactor that has been known to play a critical role in the muscular development in Drosophila. In this study, we identified and analyzed the genetic interaction of akirin with the transcriptional GATA factor, pannier. Also, we verified whether two (a third region consisting of a combination of both intronic regions) highly conserved intronic regions of akirin could be likely akirin regulatory elements by studying their expression pattern in Drosophila melanogaster. Acknowledging pannier’s role in the early stages of somatic muscular development, a genetic cross of akirin/pannier double heterozygous mutant embryos were imaged to visualize somatic muscular phenotype. Using a reporter gene in Drosophila embryos, we tested whether these identified regulatory elements are indeed akirin regulators. The results indicated that the genetic interaction of akirin and pannier is crucial for the proper development of somatic muscles and help identify the expression pattern of the potential akirin regulators in embryos.
"],"dc:identifier":["https://digitalcommons.kennesaw.edu/integrbiol_etd/88"],"dc:subject":["akirin","Biology","Integrative Biology"],"dc:title":["Interaction of akirin and pannier during embryogenesis of Drosophila melanogaster"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science in Integrative Biology (MSIB)"]},"updated_at":"2026-07-24T02:43:58Z"}