ΣΥΓΚΡΙΤΙΚΗ ΜΕΛΕΤΗ ΤΗΣ ΧΟΡΙΟΓΕΝΕΣΗΣ ΣΕ ΕΝΤΟΜΑ ΟΙΚΟΝΟΜΙΚΗΣ ΣΗΜΑΣΙΑΣ

THE INSECT EGGSHELL (CHORION AND VITELLITE MEMBRANE) AND THE OVERLAID EPITHELIUM (FOLLICLE CELLS) HAS BEEN EXTENSIVELY USED STRUCTURALLY AND MOLECULARLY AS A MODEL SYSTEM FOR STUDIES ON CELL DIFFERENTIATION. THE MOST THOROUGHLY STUDIED EGGSHELL BELONGS TO THE FRUIT FLY DROSOPHILLA MELANOGASTER (DIPTERA: DROSOPHILIDAE). THE ENZYME PEROXIDASE WAS FOUND TO BE A STRUCTURAL COMPOUND OF THE EGGSHELL AND IT IS SUPPOSED TO BE RESPONSIBLE FOR THE COVALLENT TYROSINE BONDS, BETWEEN THE EGGSHELL PROTEINS AND THE CONSECUTIVE HARDENING OF THE EGGSHELL. THE INSECTS OF ECONOMIC IMPORTANCE, STUDIED IN THE THESIS, ARE THE MEDITERRANEAN FRUIT FLY CERATITIS CAPITATA (DIPTERA: TEPHRITIDAE), THE OLIVE FLY DACUS OLEAE (DIPTERA: TEPHRITIDAE), THE CHERRY FLY RHAGOLETIS CERASI (DIPTERA: TEPHRITIDAE) AND THE ALMOND WASP EURYTOMA AMYGDALI (HYMENOPTERA: EURYTOMIDAE), AND THE MAIN GOAL SUCCEEDED IN THE THESIS WAS THE COMPARISON OF THE STRUCTURAL AND BIOCHEMICAL COUNTERPARTS OF THE EGGSHELLS OF THE ABOVE INSECTS. SEVERAL METHODS WERE USED SUCH AS: TRANSMISSION ELECTRON MICROSCOPY, SCANNING E.M., IN VITRO CULTURING OF FOLLICLES, IN VITRO LABELLING OF FOLLICLES, WITH A RADIOACTIVE AMINOACID, AUTORADIOGRAPHY WITH PULSE-CHASE EXPERIMENTS, ONE AND TWO DIMENTIONAL ELECTROPHORESIS,AUTOFLUOROGRAPHY, SILVER STAINING METHOD OF GELS, IMMUNOCALIZATION OF PEROXIDASE WITH WESTERN BLOTTING AND DOUBLE ANTIBODY STAINING, PEROXIDASE LOCALIZATION IN THE LIGHT AND ELECTRON MICROSCOPE LEVEL USING THE O-DIANISIDINE AND DIAMINOBENZIDINE (DAB) METHODS, RESPECTIVELY.

Grantor dc:publisher

National and Kapodistrian University of Athens

Year dc:date

1991

Author dc:creator

• Μουζάκη, Δέσποινα

• Δάκος της ελιάς
• ΕΝΤΟΜΑ ΟΙΚΟΝΟΜΙΚΗΣ ΣΗΜΑΣΙΑΣ
• ΕΥΡΥΤΟΜΟ ΤΗΣ ΑΜΥΓΔΑΛΙΑΣ
• ΘΥΛΑΚΟΚΥΤΤΑΡΑ
• Κέλυφος
• Μεσογειακή μύγα
• ΜΥΓΑ ΤΗΣ ΚΕΡΑΣΙΑΣ
• ΠΡΩΤΕΙΝΕΣ ΧΟΡΙΟΥ
• Υπεροξειδάσες, Ένζυμα
• Χοριογένεση
• ALMOND WASP - FOLLICLE CELLS
• CHERRY FLY
• Choriogenesis
• EGGSHELL
• EGGSHELL PROTEINS
• ENDOCHORION
• INSECTS OF ECONOMIC IMPORTANCE
• Medfly
• OLIVE FLY
• Peroxidase
• Φυσικές Επιστήμες
• Βιολογία
• Natural Sciences
• Biological Sciences

Language dc:language

gre

Identifier

10.12681/eadd/1751

OAI identifier oai:identifier

oai:10442/1751