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Showing 1 to 20 of 416 for “"Peroxidase"”.
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Unusual catalytic properties of horseradish peroxidase
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Applied Biological Sciences, 1986.
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Directed evolution of split APEX peroxidase
APEX is an engineered peroxidase that catalyzes the oxidation of a wide range of substrates, facilitating its use in a variety of applications, from subcellular staining for electron microscopy to proximity biotinylation for spatially restricted proteomics and transcriptomics. While this strategy …
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Meerrettich Peroxidase : Modifikationen und Anwendungen in Biosensoren
… und als Biokomponente das Enzym „Meerrettich Peroxidase“ (engl. horseradish peroxidase, HRP) verwendet. Solche HRP-Elektroden werden für die Messung von Wasserstoffperoxid (H2O2) eingesetzt. H2O2 wird im Körper von weißen Blutkörperchen produziert, um Bakterien abzutöten, wird teilweise …
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Treatment of 2,4-dichlorophenol using soybean peroxidase.
… presence of hydrogen peroxide (H2O2), soybean peroxidase (SBP) extracted from the soybean seed coat catalyzes the polymerization of various chlorinated phenols from wastewater. The resulting polymer precipitates from solution and can be removed through a simple sedimentation or filtration …
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Glutathione Peroxidase as an Oxidative Defense Enzyme
Made available in DSpace on 2014-12-14T15:33:40Z (GMT). No. of bitstreams: 1 8026545.pdf: 5506101 bytes, checksum: 4006284f11b892e40c45b29a3165d1fe (MD5) Previous issue date: 1980
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The Protein Redesign Approach to Modeling Manganese Peroxidase
The active site tryptophans were removed from MnCcP in order to investigate the roles of Trp51 and Trp191 in MnP activity. These residues are phenyalanines in MnP. In CcP mutants without the Mn(II)-binding site, Trp191 and Trp51 greatly influence the lifetime of the porphyrin pi-cation radical. The …
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Studies of the chlorinating intermediate of horseradish peroxidase
The chlorinating intermediate of horseradish peroxidase, Compound X, is the only know stable enzymatic halogenating intermediate known. The optical spectrum of Compound X is virtually identical to the optical spectrum of Compound II. Titration of Compound X by a single electron donor, potassium …
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Inhibition of horseradish peroxidase activity by specific antibody
… the antibody-mediated inhibition of horseradish peroxidase activity. Inhibition of enzyme activity was determined when alternate substrates were used. Typical biphasic patterns of inhibition were observed with each of five electron donors and three hydroperoxides. In no case was inhibition …
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Studies on the Heat Inactivation of Sweet Corn Peroxidase and the Relationship Between Peroxidase Activity and Quality of the Frozen Product
Made available in DSpace on 2014-12-05T19:06:44Z (GMT). No. of bitstreams: 1 5801746.pdf: 3867176 bytes, checksum: 7050f4d60e765acdbd854f930246ac25 (MD5) Previous issue date: 1958
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Heme Peroxidase Hpx-2 Protects Caenorhabditis Elegans From Pathogens
<p>Heme-containing peroxidases are important components of innate immunity. Many of them functionally associate with NADPH oxidase (NOX)/dual oxidase (DUOX) enzymes by using the hydrogen peroxide they generate in downstream reactions. In <em>Caenorhabditis elegans,</em> a ShkT-containing …
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Analysis of the peroxidase-catalysed oxidation of hydroxamic acids
… hydroxamic acids to the active site of a model peroxidase (horseradish, LPO; myeloperoxidase, MPO) was used to provide insight into structure-function relationships of peroxidases. Hydroxamic acids acted as inhibitors of the native state (E), by formation of an E-hydroxamic acid complex that …
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Probing the redox-active residues in cytochrome c peroxidase
The reaction of cytochrome c peroxidase (CCP) with H 2 O 2 results in compound I formation, where the two oxidizing equivalents of H 2 O 2 are stored as an oxyferryl heme and a Trp191 radical. Ferrocytochrome c normally reduces compound I back to the resting enzyme, but in the absence of exogenous …
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Studies on Glutathione Peroxidase of the Rat Gastrointestinal Tract
Made available in DSpace on 2015-05-12T22:36:30Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 7514151.PDF: 2915447 bytes, checksum: 37da301df0cbf65b459a4f500e74f16c (MD5) Previous issue date: 1975
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Engineering a highly enantioselective horseradish peroxidase by directed evolution
… of the commercially useful enzyme, horseradish peroxidase (HRP), toward a chiral phenol. In doing so, we discovered that mutations close to the active site not only preserve HRP catalytic activity but impact its enantioselectivity far greater than distal mutations. We thus examined how a single …
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The oxidation of tyramine and related compounds by peroxidase
Thesis (Ph.D.) Massachusetts Institute of Technology. Dept. of Biology, 1954.
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Development of a Chemiluminescent Assay for Autoantibodies to Thyroid Peroxidase
The presence of antibodies to thyroid peroxidase (i.e. TPOAb) in human serum has been measured using an immunoradiometric assay (IRMA), an enzyme-immunometric assay, and three other immunoassay systems which incorporated the chemiluminescent label acridinium ester (AE).<br/><br/>The IRMA …
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Peroxidase, Protein, and Growth Responses of Cultured Tomato Pith Explants
… semi-solid medium were analyzed for changes in peroxidase activity, protein, and growth during a seven day period. Changes in the soluble peroxidase fraction were similar to those observed previously in wounded intact tissue. Protein synthesis, the presence of a carbon-energy source, aerobic …
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Inhibition and inactivation of prostaglandin synthase-2 and horseradish peroxidase
… containing both cyclooxygenase (COX) and peroxidase (POX) active sites. Development of PGHS-2-selective POX inhibitors may reduce the toxic and carcinogenic effects of PGHS-2 xenobiotic activation, as well as further define the role of this activity in vivo . Knowledge of the contribution …
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